中文摘要：本文研究了乙二胺四乙酸（EDTA）和噬菌体对食源性病原体的联合作用。空肠弯曲菌在噬菌体PC10存在下培养8小时后活菌数下降了1.5log，但此后观察到再生长。噬菌体PC10和1mM EDTA的联用能显著抑制空肠弯曲菌的再生长，24h后空肠弯曲菌活菌数较初始计数下降2.6log；此外，0.67或1.3mM的EDTA与特异性裂解噬菌体联用，也能有效抑制大肠弯曲菌、肠炎沙门氏菌和鼠伤寒沙门氏菌的噬菌体抗性细胞的再生长。还研究了裂解噬菌体和EDTA在低温和最佳生长温度下对弯曲杆菌在BHI肉汤中生存能力的联合作用，发现1.3mM EDTA共存可显著抑制大肠杆菌的再生长，培养后的活菌数与初始活菌数基本一致。本研究首次证明了裂解噬菌体和EDTA联用可有效抑制耐噬菌体革兰氏阴性细菌的再生长。
外文摘要：The combined effects of ethylenediaminetetraacetic acid (EDTA) and bacteriophage (phage) treatment of foodborne pathogens were investigated. Although viable counts for Campylobacter jejuni decreased by 1.5 log after incubation for 8 h in the presence of phage PC10, re-growth was observed thereafter. The combination of phage PC10 and 1 mM EDTA significantly inhibited the re-growth of C. jejuni. The viable counts for C. jejuni decreased by 2.6 log (P < 0.05) compared with that of the initial count after 24 h. Moreover, EDTA at 0.67 or 1.3 mM, combined with the specific lytic phages, also effectively inhibited the re-growth of phage-resistant cells of Campylobacter coli, Salmonella enterica serovar Enteritidis, and Salmonella enterica serovar Typhimurium. In addition, the combined effects of lytic phages and EDTA were investigated on the viability of Campylobacter in BHI broth at low temperatures followed by the optimum growth temperature. The re-growth of C. coli was significantly inhibited by the coexistence of 1.3 mM EDTA, and the viable counts of surviving bacteria was about the same as the initial viable count after the incubation. This is the first study demonstrating the combined use of lytic phages and EDTA is effective in inhibiting the re-growth of phage-resistant bacteria in Gram-negative bacteria.
外文关键词：Bacteriophages；EDTA；Combination；Re-growth；Biocontrol
作者：Huang, HH；Furuta, M；Nasu, T；Hirono, M；Pruet, J；Duc, HM；Zhang, Y；Masuda, Y；Honjoh, KI；Miyamoto, T
作者单位：Kyushu Univ；Nakamura Gakuen Univ Jr Coll；Vietnam Natl Univ Agr
期刊名称：FOOD MICROBIOLOGY
期刊影响因子：4.155
出版年份：2021
出版刊次：100
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