中文摘要：病毒通过噬菌体受体结合蛋白（RBPs）来识别细菌宿主细胞上的特定表面结构。重组RBPs已用于几种病原体的检测，通常与报告酶或荧光蛋白融合。炭疽的病原体炭疽杆菌与蜡样芽孢杆菌群的其他物种关系密切，因此鉴定炭疽杆菌很困难。本文使用两种酶联噬菌体受体结合蛋白分析法（ELPRA）鉴定了炭疽杆菌。首先开发了一种单管离心分析法，简化了可疑菌落的快速分析。第二种分析方法可以从混合丛生固体（琼脂）培养基中识别复杂基质土壤衍生的可疑菌落。因此，这些试验用很短的处理时间鉴定了炭疽杆菌的营养细胞，并可能支持或确认通过聚合酶链反应等分子方法进行病原体检测。
外文摘要：Bacteriophage receptor binding proteins (RBPs) are employed by viruses to recognize specific surface structures on bacterial host cells. Recombinant RBPs have been utilized for detection of several pathogens, typically as fusions with reporter enzymes or fluorescent proteins. Identification of Bacillus anthracis, the etiological agent of anthrax, can be difficult because of the bacterium's close relationship with other species of the Bacillus cereus sensu lato group. Here, we facilitated the identification of B. anthracis using two implementations of enzyme-linked phage receptor binding protein assays (ELPRA). We developed a single-tube centrifugation assay simplifying the rapid analysis of suspect colonies. A second assay enables identification of suspect colonies from mixed overgrown solid (agar) media derived from the complex matrix soil. Thus, these tests identified vegetative cells of B. anthracis with little processing time and may support or confirm pathogen detection by molecular methods such as polymerase chain reaction.
外文关键词：bacteriophage；receptor binding protein；reporter fusions；enzyme-linked phage protein assay；ELPRA；anthrax；Bacillus anthracis
作者：Braun, P；Rupprich, N；Neif, D；Grass, G
作者单位：Bundeswehr Inst Microbiol IMB
期刊名称：VIRUSES-BASEL
期刊影响因子：3.816
出版年份：2021
出版刊次：8
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