中文摘要：大肠杆菌O157:H7能引起全球性严重疾病，如溶血性尿毒症综合征和血性腹泻。为了控制食品中的大肠杆菌O157:H7，本研究分离并鉴定了一种针对O157血清型的新型噬菌体BECP10。与其他大肠杆菌噬菌体不同，BECP10只能感染大肠杆菌O157菌株，而不会感染其他菌株。BECP10的48kbp基因组包含76个开放阅读框（ORF），其中33个推测为功能性ORF。该噬菌体不含溶原相关模块或毒素相关基因，表明该噬菌体可以彻底裂解。尾刺蛋白（TSP）序列与已报道的T1样噬菌体同源性很低，表明TSP可能与这种独特的宿主谱有关。通过吸附和受体鉴定，大肠杆菌O157:H7的特异性O-抗原残基是噬菌体感染的关键因素。该噬菌体在较宽的pH范围内对大肠杆菌O157:H7表现出较强的抗菌活性，并且很少产生噬菌体不敏感突变体。该噬菌体在汉堡肉饼上保持活力，并将大肠杆菌O157:H7降低到无法检测的水平，在低温下连续5天未出现耐药细胞。可见，噬菌体BECP10受大肠杆菌O157:H7污染的食品基质的良好生物防治剂。
外文摘要：Escherichia coli O157:H7 is a global concern that causes serious diseases, such as hemolytic uremic syndrome and bloody diarrhea. To control E. coli O157:H7 in food, a novel siphophage, BECP10, that targets the O157 serotype was isolated and characterized. Unlike other E. coli phages, BECP10 can only infect E. coli O157 strains, and thus, did not infect other strains. The 48 kbp genome of BECP10 contained 76 open reading frames (ORFs), including 33 putative functional ORFs. The phage did not contain lysogeny-related modules or toxin-associated genes, suggesting that the phage might be strictly lytic. The tail spike protein (TSP) sequence had very low homology with the reported T1-like phages, indicating that TSP might be related to this unique host spectrum. The specific O-antigen residue of E. coli O157:H7 may be a key factor for phage infection by adsorption and receptor identification. The phage exhibited strong antibacterial activity against E. coli O157:H7 over a broad pH range and showed little development of phage-insensitive mutants. The phage sustained viability on the burger patties and reduced E. coli O157:H7 to a non-detectable level without the emergence of resistant cells at low temperatures for five days. Therefore, phage BECP10 might be a good biocontrol agent for E. coli O157:H7-contaminated food matrices.
外文关键词：E. coli O157:H7；bacteriophage；phage receptor；antibacterial activity；food application
作者：Park, DW；Park, JH
作者单位：Gachon Univ
期刊名称：VIRUSES-BASEL
期刊影响因子：3.816
出版年份：2021
出版刊次：8
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