中文摘要：大肠杆菌O157:H7感染引起哺乳动物严重疾病，并在全球造成巨大的经济损失。由于抗生素耐药性的发展，噬菌体疗法已成为控制O157:H7感染的一种替代方法。然而，噬菌体对胃肠道环境的抗性较低，其治疗效果往往令人失望。为了提高噬菌体在胃肠道中的稳定性，本研究对大肠杆菌O157:H7噬菌体进行了微囊化，并研究了其体外稳定性和体内治疗效果。结果表明，与游离噬菌体相比，微囊化噬菌体对模拟胃液和胆盐的抗性显著增强。微囊化噬菌体被有效释放到模拟肠液中，对感染大肠杆菌O157:H7的大鼠具有比游离噬菌体更好的治疗效果。此外，微囊化噬菌体在储存期间比游离噬菌体更稳定，表明噬菌体微囊化在噬菌体治疗中可以发挥重要作用。
外文摘要：Escherichia coli (E. coli) O157:H7 bacterial infection causes severe disease in mammals and results in substantial economic losses worldwide. Due to the development of antibiotic resistance, bacteriophage (phage) therapy has become an alternative to control O157:H7 infection. However, the therapeutic effects of phages are frequently disappointing because of their low resistance to the gastrointestinal environment. In this study, to improve the stability of phages in the gastrointestinal tract, E. coli O157:H7 phages were microencapsulated and their in vitro stability and in vivo therapeutic efficiency were investigated. The results showed that compared to free phages, the resistance of microencapsulated phages to simulated gastric fluid and bile salts significantly increased. The microencapsulated phages were efficiently released into simulated intestinal fluid, leading to a better therapeutic effect in rats infected with E. coli O157:H7 compared to the effects of the free phages. In addition, the microencapsulated phages were more stable during storage than the free phages, showing how phage microencapsulation can play an essential role in phage therapy.
外文关键词：E. coli O157:H7；microencapsulated phage；antibiotic resistance；therapeutic efficiency
作者：Yin, HJ；Li, J；Huang, HS；Wang, YX；Qian, XJ；Ren, JL；Xue, F；Dai, JJ；Tang, F
作者单位：Nanjing Agr Univ；China Pharmaceut Univ
期刊名称：VETERINARY RESEARCH
期刊影响因子：3.357
出版年份：2021
出版刊次：1
点击下载：微囊化噬菌体在胃肠道环境中具有较长的稳定性，对大肠杆菌O157:H7感染具有较高的治疗效率