中文摘要：抗生素耐药基因（ARG）是一种新兴污染物，对全球人类健康构成潜在威胁。ARGs通过移动遗传元件（MGE），包括插入序列（ISs）、质粒和噬菌体，转移到其他细菌的程度与它们作为耐药性转移决定因素的可能性密切相关。因此，了解MGEs的结构和功能对于评估未来在强抗生素压力环境下与ARG相关的健康风险至关重要。本研究使用MinION和HiSeq平台进行了全基因组测序，以检测从韩国济州岛渔场污水中分离的四种多药耐药细菌的抗生素耐药性决定因素。用综合数据确定ARG和MGE之间的关联。使用HiSeq和MinION读取的杂交集合显示存在IncFIB（K）和pVPH2质粒，其大小通过脉冲场凝胶电泳进行验证。在这些质粒上注释的955个编码序列中，鉴定出24个ARG和95个MGE。更重要的是，24个对各种抗生素产生耐药性的ARG中有22个位于MGEs附近，而大约一半的ARG（21个ARG中的11个）位于染色体上。本研究从基因组学的角度揭示了在受到选择压力的细菌中可能存在的可移动ARGs的起源。
外文摘要：Antibiotic resistance genes (ARGs) are now viewed as emerging contaminants posing a potential worldwide human health risk. The degree to which ARGs are transferred to other bacteria via mobile genetic elements (MGEs), including insertion sequences (ISs), plasmids, and phages, has a strong association with their likelihood to function as resistance transfer determinants. Consequently, understanding the structure and function of MGEs is paramount to assessing future health risks associated with ARGs in an environment subjected to strong antibiotic pressure. In this study we used whole genome sequencing, done using MinION and HiSeq platforms, to examine antibiotic resistance determinants among four multidrug resistant bacteria isolated from fish farm effluent in Jeju, South Korea. The combined data was used to ascertain the association between ARGs and MGEs. Hybrid assembly using HiSeq and MinION reads revealed the presence of IncFIB(K) and pVPH2 plasmids, whose sizes were verified using pulsed field gel electrophoresis. Twenty four ARGs and 95 MGEs were identified among the 955 coding sequences annotated on these plasmids. More importantly, 22 of 24 ARGs conferring resistance to various antibiotics were found to be located near MGEs, whereas about a half of the ARGs (11 out of 21) were so in chromosomes. Our results also suggest that the total phenotypic resistance exhibited by the isolates was mainly contributed by these putatively mobilizable ARGs. The study gives genomic insights into the origins of putatively mobilizable ARGs in bacteria subjected to selection pressure.
外文关键词：Mobile genetic elements；Multidrug resistance；Mobilizable ARGs；Hybrid ssembly
作者：Farooq, A；Kim, J；Raza, S；Jang, J；Han, D；Sadowsky, MJ；Unno, T
作者单位：eju Natl Univ；Jeonbuk Natl Univ；Gangneung Wonju Natl Univ；Univ Minnesota
期刊名称：NVIRONMENTAL POLLUTION
期刊影响因子：6.793
出版年份：2021
出版刊次：
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