中文摘要：噬菌体疗法在医学中的作用最近重新得到了重视。口服噬菌体治疗胃肠道疾病、经胃转运和十二指肠快速释放就是此类应用之一。本研究提出了一种高效的聚HIPE/水凝胶系统，用于靶向递送噬菌体并在目标位置快速释放。用低交联阴离子纳米纤维素水凝胶包裹T7噬菌体，在pH<3.9（胃）时成功保护噬菌体，在pH高于3.9（十二指肠）时完全失去水凝胶网状系统，释放噬菌体。将含有噬菌体的水凝胶交联在平均直径为24μm的高孔球形聚HIPE颗粒内。聚HIPE支架保护水凝胶在运输过程中免受机械刺激，防止水凝胶结构崩塌和不必要的噬菌体释放。另一方面，由于小尺寸颗粒较大的表面体积比，能够在目标位置快速释放。因此，实现了快速零级释放，提高了患者依从性，减少了给药频率。可见，该系统在医学和药剂学中显示出靶向给药的巨大潜力。
外文摘要：The role of bacteriophage therapy in medicine has recently regained an important place. Oral phage delivery for gastrointestinal treatment, transport through the stomach, and fast release in the duodenum is one of such applications. In this work, an efficient polyHIPE/hydrogel system for targeted delivery of bacteriophages with rapid release at the target site is presented. T7 bacteriophages were encapsulated in low crosslinked anionic nanocellulose-based hydrogels, which successfully protected phages at pH < 3.9 (stomach) and completely lost the hydrogel network at a pH above 3.9 (duodenum), allowing their release. Hydrogels with entrapped phages were crosslinked within highly porous spherical polyHIPE particles with an average diameter of 24 mu m. PolyHIPE scaffold protects the hydrogels from mechanical stimuli during transport, preventing the collapse of the hydrogel structure and the unwanted phage release. On the other hand, small particle size, due to the large surface-to-volume ratio, enables rapid release at the target site. As a consequence, a fast zero-order release was achieved, providing improved patient compliance and reduced frequency of drug administration. The proposed system therefore exhibits significant potential for a targeted drug delivery in medicine and pharmacy.
外文关键词：TEMPO nanocellulose；T7 bacteriophage；encapsulation；drug diffusion；zero-order release
作者：Kopac, T；Lisac, A；Mravljak, R；Rucigaj, A；Krajnc, M；Podgornik, A
作者单位：Univ Ljubljana；COBIK
期刊名称：POLYMERS
期刊影响因子：3.426
出版年份：2021
出版刊次：16
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