中文摘要：耐多药（MDR）细菌对全球健康构成严重威胁，可通过噬菌体疗法加以解决。测定细菌对噬菌体的敏感性很重要。本研究使用带有微流控通道的微机械传感器进行快速抗菌药物敏感性测试（AST）。微流控通道的内表面通过分子连接物进行功能化，以捕获通道内的细菌细胞。大肠杆菌对噬菌体PhiX174产生反应，而金黄色葡萄球菌对噬菌体Phi44AHJD产生反应。微机械传感器测量了细菌纳米运动引起的机械波动，反映了病毒感染前后细菌的代谢活动。换句话说，微通道的时间依赖性振荡可以与细胞代谢产生的细胞膜运动相关。两种细菌的初始波动幅度不同。细菌暴露于噬菌体诱导传感器响应急剧降低，表明细菌代谢活性显著降低。与大肠杆菌相比，金黄色葡萄球菌对Phi44AHJD具有耐药性，其代谢活性的显著降低可能归因于病毒相互作用引起的应激导致的短暂休眠。该技术提供了一种快速、准确的评估细菌对150微微升小体积菌噬菌体敏感性的方法。
外文摘要：Multidrug-resistant (MDR) bacteria pose a serious threat to global health, which may be addressed using bacteriophage therapy. It is important to measure the susceptibility of bacteria to bacteriophages. We used a micromechanical sensor with a microfluidic channel for rapid antimicrobial susceptibility testing (AST). The interior surface of the microfluidic channel was functionalized with a molecule linker to capture the bacterial cells inside the channel. The Escherichia coli response to the bacteriophage PhiX174 and Staphylococcus aureus response to the bacteriophage Phi44AHJD is observed. The micromechanical sensor measured the mechanical fluctuations induced by nano-movements of the bacteria, reflecting their metabolic activity, before and after viral infection. In other words, the time-dependent oscillations of the microchannel can be correlated to the membrane movement in the cells produced by the cell metabolisms. The initial magnitudes of the fluctuations were different in the two bacteria. Bacteria exposure to the bacteriophages induced a sharp reduction of the sensor response, indicating a marked decrease in bacterial metabolic activities. In contrast to E. coli, S. aureus was resistant to Phi44AHJD, and the significant reduction in its metabolic activity may be attributed to transient dormancy due to stress caused by the viral interaction. This technique provides a rapid and accurate assessment of the susceptibility of bacteria to bacteriophages in a small volume of 150 picolitres.
作者：Alzahrani, KE；Alodhayb, A；Algwati, M；Alanazi, AF；Ain, QT；Assaifan, AK；Manoharadas, S；Alshammari, A；Alswieleh, A；Albrithen, H
作者单位：King Saud Univ
期刊名称：JOURNAL OF THE ELECTROCHEMICAL SOCIETY
期刊影响因子：3.721
出版年份：2021
出版刊次：8
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