中文摘要：细菌细胞的聚合体，嵌在自产的细胞外聚合物基质中形成细菌生物膜。生物膜的形成一直是水下测量传感器面临的主要挑战，也是测量误差的主要来源，尤其是在长期现场监测方面。本研究展示了溶解性噬菌体（细菌病毒）作为一种非侵入性策略去除电化学溶解氧传感器气体渗透膜上形成的细菌生物膜的效用。结果表明， 4天的铜绿假单胞菌基质充分发育，形成的生物膜显著影响传感器信号和响应时间，信号降低32%，响应时间增加94%。此外，和干净的传感器膜相比，生物污染膜的测量结果具有非常低的信噪比。单剂量隔夜噬菌体处理可有效去除生物膜，无需重复处理。此外，完全生物膜污染的传感器信号下降了32%，噬菌体处理后恢复到原始值（7.96+/-0.27 mg L-1），噬菌体处理膜的信噪比（计算为平均值与标准偏差之比）比生物污染膜增加了8倍。表明溶解氧传感器几乎完全再生、信号恢复，使生物污染传感器可重复使用，而无需使用可能破坏脆弱传感器膜的苛刻化学品。
外文摘要：Bacterial biofilms are aggregates of bacterial cells embedded in a self-produced extracellular polymeric matrix. Biofilm formation has always been considered a major challenge for sensors used in underwater measurements, and is a primary source of measurement error, especially when it comes to long-term in situ monitoring. We demonstrate the utility of lytic bacteriophages (bacterial viruses) as a non-invasive strategy for removing bacterial biofilms formed on the gas permeable membrane of electrochemical dissolved oxygen sensors. Our results show that a 4 day Pseudomonas aeruginosa biofilm with a fully developed matrix significantly affected the sensor signal and response time, decreasing the signal by 32% and increasing the response time by 94%. In addition, measurements with the biofouled membrane had a very low signal to nose ratio compared to a clean sensor membrane. A single dose of overnight phage treatment effectively removed the biofilm (as indicated by scanning electron micrographs and fluorescence images of the membrane), without the need for repeated treatments. Furthermore, the sensor signal that had plummeted by 32% for a fully biofouled membrane, was returned to the original value (7.96 +/- 0.27 mg L-1) after phage treatment and the signal to noise ratio (calculated as the ratio of mean to standard deviation) increased 8 folds for a phage-treated membrane compared to a biofouled membrane. Our data indicate near complete regeneration and signal recovery for the dissolved oxygen sensor, making the biofouled sensor reusable without the use of harsh chemicals that could destroy the fragile sensor membrane.
作者：Bayat, F；Maddiboina, D；Didar, TF；Hosseinidoust, Z
作者单位：McMaster Univ
期刊名称：RSC ADVANCES
期刊影响因子：3.119
出版年份：2021
出版刊次：14
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