中文摘要：噬菌体感染是牛奶发酵中的一个严重问题，它会导致发酵过程延迟，降低产品质量。本研究首次提供了泰国乳制品行业从变质发酵乳罐中提取的乳酸噬菌体的分子特征。噬菌体Phi T25具有毒力，对副干酪乳杆菌LPC具有较强的裂解活性。基因组分析表明，噬菌体Phi T25具有38421 bp的线性双链DNA基因组。为了检测噬菌体Phi T25（ORF35）的内溶素活性，通过将细胞裂解酶T25基因克隆到质粒pET21a中，构建了细胞裂解酶载体pET2la。推测细胞裂解酶T25内溶素的大小约41kDa。细胞裂解酶T25能够裂解多种乳酸菌和其他革兰氏阳性菌，如金黄色葡萄球菌和单核细胞增生李斯特菌。此外，该内溶素与氯仿作为外膜透化剂联合使用时，对大肠杆菌具有协同抑制作用。
外文摘要：Bacteriophage infection is a serious problem in milk fermentation where it causes a delay in the fermentation process and reduces product quality. This study provides the first molecular characterisation of a lactic acid bacteriophage from spoiled fermented milk tanks in the dairy industry in Thailand. Bacteriophage Phi T25 is virulent, and shows strong lytic activity against Lacticaseibacillus paracasei LPC. Genome analysis demonstrated that phage Phi T25 possesses a linear, double-stranded DNA genome of 38,421 bp. To assay endolysin activity of phage Phi T25 (ORF35), Lysin vector pET2la was constructed by cloning the LysinT25 gene into plasmid pET21a. The size of LysinT25 endolysin was predicted to be similar to 41 kDa LysinT25 was able to lyse a wide range of lactic acid bacteria and other Gram-positive bacteria, such as Staphylococcus aureus and Listeria monocytogenes. In addition, this endolysin has a synergistic inhibitory effect against Escherichia coli when combined with chloroform as an outer-membrane permeabiliser.
作者：Sunthornthummas, S；Doi, K；Fujino, Y；Rangsiruji, A；Sarawaneeyaruk, S；Insian, K；Pringsulaka, O
作者单位：Srinakharinwirot Univ；Mahidol Univ；Kyushu Univ
期刊名称：INTERNATIONAL DAIRY JOURNAL
期刊影响因子：2.512
出版年份：2021
出版刊次：
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