中文摘要：病毒性传染病的不断出现已成为公共卫生的主要威胁。为了量化病毒，需要正确处理水样，以确保测试结果的准确性和可靠性。本研究开发了增强型多孔高吸水性聚合物（PSAP）微球，用于病毒检测水样的预处理及储存。通过应用PSAP微球收集水样，病毒被捕获并封装在微球内，而不需要的成分被排除。研究成功地证明了模型病毒在室温（22℃）和高温（35℃）下可以有效延长保质期。即使在未经处理的废水等复杂介质中，病毒的传染性水平和基因组丰度也得以保存。在测试条件下，使用PSAP微球可将病毒降解速率常数降低到10倍以上。因此，增强型PSAP微球提供了一种低成本、高效的样品预处理和储存方法，可用于大规模监测水样中的病毒病原体。
外文摘要：The continuous emergence of infectious viral diseases has become a major threat to public health. To quantify viruses, proper handling of water samples is required to ensure the accuracy and reliability of the testing results. In this study, we develop enhanced porous superabsorbent polymer (PSAP) beads to pretreat and store water samples for virus detection. By applying PSAP beads to collect water samples, the viruses are captured and encapsulated inside the beads while undesired components are excluded. We have successfully demonstrated that the shelf life of the model virus can be effectively extended at room temperature (22 degrees C) and an elevated temperature (35 degrees C). Both the infectivity level and genome abundance of the viruses are preserved even in a complex medium such as untreated wastewater. Under the tested conditions, the viral degradation rate constant can be reduced to more than 10 times using the PSAP beads. Therefore, the enhanced PSAP beads provide a low-cost and efficient sample pretreatment and storage method that is feasible and practical for large-scale surveillance of viral pathogens in water samples.
外文关键词：porous polymer；separation；encapsulation；stabilization；virus surveillance
作者：Chen, WS；Wang, T；Dou, ZO；Xie, X
作者单位：Georgia Inst Technol
期刊名称：ENVIRONMENTAL SCIENCE & TECHNOLOGY
期刊影响因子：7.864
出版年份：2021
出版刊次：20
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