中文摘要：噬菌体及其细菌宿主是地球上最丰富、遗传多样性最高的生物。本研究从溶藻弧菌中分离出一系列噬菌体抗性突变株（PRM），并采用罕见限制性位点PCR（IRS-PCR）对PRM菌株的遗传多样性进行了研究。通过碳源利用谱和化学敏感性分析了8个PRM菌株的表型变异。采用全基因组测序和重测序，分别分析了8株PRM菌株和与噬菌体共进化的溶藻弧菌群体的遗传变异。结果表明，8个遗传差异的PRM菌株表现出丰富的表型变异。八个PRM菌株和共同进化的溶藻弧菌群体（VE1、VE2和VE3）包含大量单核苷酸变异（SNV）和插入/缺失（INDEL），并表现出明显的遗传分化。编码基因中大多数SNV和INDEL与通常作为噬菌体入侵受体的鞭毛、胞外多糖（EPS）的合成有关。PRM菌株和共同进化的细胞群也含有tRNA和rRNA基因的频繁突变。三分之二的共同进化群体(VE1和VE2)包含一个大的突变片段，严重解构了基因nrdA，这是基因组突变率激增的可预测的原因。总之，在溶藻弧菌PRM菌株的基因组和噬菌体感染应激下溶藻弧菌的共进化细胞群中检测到许多突变和遗传分化，噬菌体感染应激可能是驱动溶藻弧菌基因组进化的重要因素。
外文摘要：Bacteriophages (phages) and their bacterial hosts were the most abundant and genetically highly diverse organisms on the earth. In this study, a series of phage-resistant mutant (PRM) strains derived from Vibrio alginolyticus were isolated and Infrequent-restriction-site PCR (IRS-PCR) was used to investigate the genetic diversity of the PRM strains. Phenotypic variations of eight PRM strains were analyzed using profiles of utilizing carbon sources and chemical sensitivity. Genetic variations of eight PRM strains and coevolved V. alginolyticus populations with phages were analyzed by whole-genome sequencing and resequencing, respectively. The results indicated that eight genetically discrepant PRM stains exhibited abundant and abundant phenotypic variations. Eight PRM strains and coevolved V. alginolyticus populations (VE1, VE2, and VE3) contained numerous single nucleotide variations (SNVs) and insertions/indels (InDels) and exhibited obvious genetic divergence. Most of the SNVs and InDels in coding genes were related to the synthesis of flagellar, extracellular polysaccharide (EPS), which often served as the receptors of phage invasion. The PRM strains and the coevolved cell populations also contained frequent mutations in tRNA and rRNA genes. Two out of three coevolved populations (VE1 and VE2) contained a large mutation segment severely deconstructing gene nrdA, which was predictably responsible for the booming of mutation rate in the genome. In summary, numerous mutations and genetic divergence were detected in the genomes of V. alginolyticus PRM strains and in coevolved cell populations of V. alginolyticus under phage infection stress. The phage infection stress may provide an important force driving genomic evolution of V. alginolyticus.
外文关键词：Vibrio alginolyticus；phages；genetic divergence；phage-resistant mutations；genomic changes
作者：Zhou, WJ；Li, YY；Li, ZB；Ma, B；Jiang, X；Hu, CQ；Ai, YX；Luo, P
作者单位：Jilin Univ；Chinese Acad Sci；Southern Marine Sci & Engn Guangdong Lab Guangzho；Geol Survey Inst Guangzhou；Univ Chinese Acad Sci
期刊名称：FRONTIERS IN MICROBIOLOGY
期刊影响因子：4.236
出版年份：2021
出版刊次：
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