中文摘要：阳离子肽嵌合细胞裂解酶Lysqdvp001-5aa、Lysqdvp001-10aa和Lysqdvp001-15aa是基于副溶血性弧菌噬菌体qdvp001中的细胞裂解酶Lysqdvp001设计的。这些嵌合细胞裂解酶显示出与Lysqdvp001相当的肽聚糖水解活性，并能从外部裂解细菌。Lysqdvp001和嵌合细胞裂解酶对副溶血性弧菌的抗菌活性及内外膜通透性为Lysqdvp001-15aa>Lysqdvp001-10aa>Lysqdvp001-5aa>Lysqdvp001。Lysqdvp001-15aa显示出优异的抗菌活性，最低抑菌和杀菌浓度（MIC和MBC）分别为0.2和0.4mg/mL，其抗菌谱比噬菌体qdvp001更宽。Lysqdvp001-15aa处理后，出现副溶血弧菌的膜超极化和膜磷脂暴露。透射电镜（TEM）显示Lysqdvp001-15aa破坏了副溶血性弧菌的结构完整性。此外，Lysqdvp001-15aa的MIC和MBC使牡蛎中的副溶血性弧菌数量分别减少了3.20和4.03 log10CFU/g。MBC的Lysqdvp001-15aa可根除约50%的副溶血性弧菌生物膜，并抑制90%以上细菌生物膜的形成。
外文摘要：Cationic peptide chimeric lysins, Lysqdvp001-5aa, Lysqdvp001-10aa and Lysqdvp001-15aa, were designed based on lysin Lysqdvp001 from Vibrio parahaemolyticus (V. parahaemolyticus) phage qdvp001. These chimeric lysins showed equivalent peptidoglycan hydrolysis activities with Lysqdvp001 and could lyse the bacteria from the outside. The antibacterial activity as well as outer and inner membrane permeabilization of Lysqdvp001 and chimeric lysins against V. parahaemolyticus were Lysqdvp001-15aa>Lysqdvp001-10aa>Lysqdvp001-5aa>Lysqdvp001. Lysqdvp001-15aa exhibited an excellent antibacterial activity with minimum inhibition and bactericidal concentrations (MIC and MBC) of 0.2 and 0.4 mg/mL, respectively, and its antibacterial spectrum was much broader than phage qdvp001. Membrane hyperpolarization and membrane phospholipid exposure of V. parahaemolyticus were observed after Lysqdvp001-15aa treatments. Transmission electron microscope (TEM) showed Lysqdvp001-15aa destroyed structure integrity of V. parahaemolyticus. Besides, MIC and MBC of Lysqdvp001-15aa decreased V. parahaemolyticus counts in oyster by 3.20 and 4.03 log10CFU/g. Lysqdvp001-15aa at MBC eradicated about 50% of V. parahaemolyticus biofilms and inhibited over 90% of the formation of the bacterial biofilms.
外文关键词：Chimeric lysin；Cationic peptide；Vibrio parahaemolyticus；Biofilm
作者：Ning, HQ；Cong, Y；Lin, H；Wang, JX
作者单位：Ocean Univ China
期刊名称：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
期刊影响因子：4.187
出版年份：2021
出版刊次：
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