中文摘要：：Enteric-coated bacteriophage tablets for oral administration against gastrointestinal infections
中文摘要：肠道铜绿假单胞菌在免疫功能低下患者（如在医院重症监护病房的患者）中引发严重问题。噬菌体疗法是一种有吸引力的抗生素替代或补充疗法，因为它不仅能杀死耐多药细菌，还能最大限度地减少肠道菌群的破坏。固体口服剂型（即片剂）可有效地将活噬菌体运送到胃肠道，但配方研究很少。本研究以假单胞菌靶向噬菌体PEV20为模型，利用工业规模的压片机和包衣机生产适合口服的片剂。噬菌体片剂制备包括（i）噬菌体喷雾干燥，（ii）将噬菌体粉末直接压缩成片剂，（iii）片剂包衣。整个过程中，噬菌体片剂的噬菌体滴度降低可忽略不计，并通过了英国药典测试，包括片剂的脆性、重量变化、崩解和溶解，以及包衣片剂的重量增加和崩解（在0.1 M HCl和pH 7.4磷酸盐缓冲液中）。该配方方法可用于生产含有其他噬菌体和噬菌体混合物的片剂，有效地对抗肠道细菌感染。
外文摘要：Intestinal Pseudomonas aeruginosa is highly problematic in immunocompromised patients such as those in intensive care units in hospitals. Phage therapy is an attractive alternative or supplementary therapy to antibiotics as it not only kills multidrug-resistant bacteria, but also minimises the disruption of gut microflora. Solid oral dosage forms (i.e., tablets) have the potential to effectively deliver viable phages to the gastrointestinal tract, but formulation studies have been scarce. In this study, Pseudomonas-targeting phage PEV20 was used as a model to produce tablets suitable for oral delivery by utilising industry-scale tablet compression and tablet coating machines. Phage tablets were produced by (i) spray drying of phages, (ii) direct compression of the phage powders into tablets, and then (iii) tablet coating. The resulting phage tablets had negligible phage titre reduction throughout the process and passed the British Pharmacopeia tests, including friability, weight variation, disintegration and dissolution of the tablets as well as weight gain and disintegration (in 0.1 M HCl and pH 7.4 phosphate buffer) of coated tablets. The developed formulation method can be utilised to produce tablets containing other phages and phage cocktails that are effective against enteric bacterial infections.
外文关键词：Bacteriophages (phages)；Pseudomonas aeruginosa；Bacteriophage therapy；Tablet；Spray dry；Direct compression；Enteric coating
作者：Khanal, D；Chang, RYK；Hick, C；Morales, S；Chan, HK
作者单位：Univ Sydney
期刊名称：INTERNATIONAL JOURNAL OF PHARMACEUTICS
期刊影响因子：4.845
出版年份：2021
出版刊次：
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