中文摘要：免疫系统的任务是保护身体不受伤害，保持健康。在免疫系统中，B淋巴细胞和T淋巴细胞是两种主要成分，共同阻止和消除病毒颗粒、细菌、真菌和寄生虫等入侵威胁。与识别主要组织相容性复合体（MHC）上抗原肽的T细胞受体（TCR）相比，抗体在目标识别中的功能相对更直接。尽管噬菌体展示已广泛应用于抗体呈递，但TCR的情况却恰恰相反。细胞表面TCR是一个相对较大且复杂的分子，因此很难在噬菌体表面展示。可即便如此，依旧引入了重组和修饰，以便TCR在噬菌体展示中不断发展。此外，TCR在免疫治疗中的应用日益广泛，已成为噬菌体展示法开发的重要结合基序。本文着重介绍噬菌体展示技术在TCR发现及TCR工程方面的应用。
外文摘要：The immune system is tasked to keep our body unharmed and healthy. In the immune system, B- and T-lymphocytes are the two main components working together to stop and eliminate invading threats like virus particles, bacteria, fungi and parasite from attacking our healthy cells. The function of antibodies is relatively more direct in target recognition as compared to T-cell receptors (TCR) which recognizes antigenic peptides being presented on the major histocompatibility complex (MHC). Although phage display has been widely applied for antibody presentation, this is the opposite in the case of TCR. The cell surface TCR is a relatively large and complex molecule, making presentation on phage surfaces challenging. Even so, recombinant versions and modifications have been introduced to allow the growing development of TCR in phage display. In addition, the increasing application of TCR for immunotherapy has made it an important binding motif to be developed by phage display. This review will emphasize on the application of phage display for TCR discovery as well as the engineering aspect of TCR for improved characteristics.
外文关键词：T-cell receptor；Phage display；Major histocompatibility complex；Panning；single chain T-cell receptor
作者：Ch'ng, ACW；Lam, P；Alassiri, M；Lim, TS
作者单位：Univ Sains Malaysia；CellVec Private Ltd；Natl Univ Singapore；Duke NUS Grad Med Sch；King Saud Bin Abdulaziz Univ Hlth Sci KSAU HS；King Abdullah Int Med Res Ctr KAIMRC；Univ Sains Malaysia
期刊名称：BIOTECHNOLOGY ADVANCES
期刊影响因子：10.744
出版年份：2021
出版刊次：
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