中文摘要：本文提出了一种新的基于Au/CaCO3的超灵敏电化学发光（ECL）免疫分析方法，用于检测咖啡中的赭曲霉毒素A（OTA）。以废弃蛋壳为模板合成的Au/CaCO3纳米复合材料具有较大的表面积和优异的电化学性能，用于固定大量Ru（bpy）（3）（2+）和共轭高亲和力纳米体（通过噬菌体展示技术制备）。Au/CaCO3纳米复合材料和纳米体技术的耦合为OTA检测提供了一种超灵敏、高选择性的ECL免疫传感器，检测范围为10 pg/mL-100 ng/mL，低检测限为5.7 pg/mL；所制备的ECL免疫传感器具有良好的性能和较高的稳定性；将该ECL传感器应用于咖啡样品中OTA的分析，证实了理想的精度和实用潜力。总之，本文提出的新纳米材料，通过利用天然废弃物为来源制造免疫传感器的传感界面，并提出了一种检测食品中有毒OTA的方法。
外文摘要：A novel ultrasensitive electrochemiluminescence (ECL) immunoassay based on Au/CaCO3 was proposed for detecting ochratoxin A (OTA) in coffee. Au/CaCO3 nanocomposites synthesized using waste eggshells as the template with a large surface area and excellent electrochemical properties were applied for immobilizing a large amount of Ru(bpy)(3)(2+) and conjugating a high-affinity nanobody (prepared by the phage display technique). Coupling of the Au/CaCO3 nanocomposites and nanobody technologies provided an ultrasensitive and highly selective ECL immunosensor for OTA detection in the range of 10 pg/mL-100 ng/mL with a low detection limit of 5.7 pg/mL. Moreover, the as-prepared ECL immunosensor showed excellent performance and high stability. Finally, the proposed ECL sensor was applied to analyze OTA in coffee samples, confirming the desirable accuracy and practical applicability potential. Overall, this work presents a new nanomaterial for fabricating the sensing interface of immunosensors by harnessing natural waste as the source and a method for detecting toxic OTA in foods.
作者：Li, LZ；Liu, X；He, SJ；Cao, HM；Su, BC；Huang, TZ；Chen, Q；Liu, MH；Yang, DP
作者单位：Hainan Univ；Quanzhou Normal Univ；Ludong Univ
期刊名称：ACS OMEGA
期刊影响因子：2.87
出版年份：2021
出版刊次：44
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