中文摘要：肺炎克雷伯菌是一种革兰氏阴性菌，已成为威胁生命的医疗相关感染（HAI），包括肺炎和败血症的主要病因之一。此外，由于其日益增加的抗生素耐药性，肺炎克雷伯菌已成为全球最优先关注的问题。肺炎克雷伯菌感染问题部分是由于无法快速准确地检测病原体，从而无法在感染早期开展治疗。细菌检测的成功在很大程度上取决于所使用的生物识别分子，目前的诊断工具依赖于昂贵的探针，通常缺乏特异性和/或敏感性。噬菌体受体结合蛋白（RBPs）负责噬菌体对特定细菌宿主受体的识别和吸附，用作生物识别分子具有很高的潜力。本研究从肺炎克雷伯菌噬菌体KpnM6E1中鉴定了一种新型RBP，该RBP对目标菌具有高特异性，对肺炎克雷伯菌具有高敏感性(80%)。此外，吸附研究证实了gp86在细菌受体吸附中的作用：高度抑制(86%)噬菌体对其克雷伯氏菌宿主的吸附。总之，本研究揭示了新型克雷伯菌噬菌体RBP的强大作用和潜力，可与分析技术或生物传感平台结合使用，诊断肺炎克雷伯菌感染。
外文摘要：Klebsiella pneumoniae is a Gram-negative bacterium that has become one of the leading causes of life-threatening healthcare-associated infections (HAIs), including pneumonia and sepsis. Moreover, due to its increasingly antibiotic resistance, K. pneumoniae has been declared a global top priority concern. The problem of K pneumoniae infections is due, in part, to the inability to detect this pathogen rapidly and accurately and thus to treat patients within the early stages of infections. The success in bacterial detection is greatly dictated by the biorecognition molecule used, with the current diagnostic tools relying on expensive probes often lacking specificity and/or sensitivity. (Bacterio)phage receptor-binding proteins (RBPs) are responsible for the recognition and adsorption of phages to specific bacterial host receptors and thus present high potential as biorecognition molecules. In this study, we report the identification and characterization of a novel RBP from the K. pneumoniae phage KpnM6E1 that presents high specificity against the target bacteria and high sensitivity (80%) to recognize K. pneumoniae strains. Moreover, adsorption studies validated the role of gp86 in the attachment to bacterial receptors, as it highly inhibits (86%) phage adsorption to its Klebsiella host. Overall, in this study, we unravel the role and potential of a novel Klebsiella phage RBP as a powerful tool to be used coupled with analytical techniques or biosensing platforms for the diagnosis of K. pneumoniae infections.
外文关键词：bacteriophage；receptor-binding protein (RBP)；Klebsiella pneumoniae； diagnosis；hospital acquired infections (HAIs)
作者：Nogueira, CL； Pires, DP；Monteiro, R；Santos, SB；Carvalho, CM
作者单位：Int Iberian Nanotechnol Lab INL；Inst Engn Sistemas & Computadores Microsistemas &；Univ Minho
期刊名称：ACS INFECTIOUS DISEASES
期刊影响因子：4.614
出版年份：2021
出版刊次：11
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