中文摘要：金黄色葡萄球菌能在细胞外聚合物基质中形成多层生物膜。这种生物膜难以去除，对抗生素不敏感，容易产生耐药菌株，并对环境和健康造成巨大问题。噬菌体细胞裂解酶通常由催化结构域（CD）和细胞壁结合结构域（CBD）组成，是对抗细菌生物膜的有力武器。但细胞裂解酶和金黄色葡萄球菌生物膜之间的实时相互作用仍不甚清楚。本研究基于表面等离子体共振（SPR）实时监测了三种细胞裂解酶（ClyF、ClyC、PlySs2）与芯片培养金黄色葡萄球菌生物膜的相互作用。典型的SPR反应曲线表明，细胞裂解酶迅速与生物膜结合，对生物膜破坏的时间更长。通过1:1结合模型分析，发现ClyF、ClyC和PlySs2的亲和力常数（K-D）分别为3.18+/-0.127μM、1.12+/-0.026μM和15.5+/-0.514μM。ClyF和PlySs2具有相同的CBD，但与金黄色葡萄球菌生物膜的亲和力不同，表明不仅CBD，CD也影响整个细胞裂解酶的结合活性。SPR可以提高对细胞裂解酶和细菌生物膜之间，包括结合（吸附）和解离（破坏）在内的复杂相互作用的理解。
外文摘要：Staphylococcus aureus can produce a multilayered biofilm embedded in extracellular polymeric matrix. This biofilm is difficult to remove, insensitive to antibiotics, easy to develop drug-resistant strains and causes enormous problems to environments and health. Phage lysin which commonly consists of a catalytic domain (CD) and a cell-wall binding domain (CBD) is a powerful weapon against bacterial biofilm. However, the real-time interaction between lysin and S. aureus biofilm is still not fully understood. In this study, we monitored the interactions of three lysins (ClyF, ClyC, PlySs2) against culture-on-chip S. aureus biofilm, in real-time, based on surface plasmon resonance (SPR). A typical SPR response curve showed that the lysins bound to the biofilm rapidly and the biofilm destruction started at a longer time. By using 1:1 binding model analysis, affinity constants (K-D) for ClyF, ClyC, and PlySs2 were found to be 3.18 +/- 0.127 mu M, 1.12 +/- 0.026 mu M, and 15.5 +/- 0.514 mu M, respectively. The fact that ClyF and PlySs2 shared the same CBD but showed different affinity to S. aureus biofilm suggested that, not only CBD, but also CD affects the binding activity of the entire lysin. The SPR platform can be applied to improve our understanding on the complex interactions between lysins and bacterial biofilm including association (adsorption) and disassociation (destruction).
外文关键词：real-time；S；aureus；biofilms；lysins；CBD；CD；SPR；affinity
作者：Hong, W；Nyaruaba, R；Li, XH；Liu, H；Yang, H；Wei, HP
作者单位：Chinese Acad Sci；Univ Chinese Acad Sci
期刊名称：FRONTIERS IN MICROBIOLOGY
期刊影响因子：4.236
出版年份：2021
出版刊次：
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