中文摘要：植物中的病毒侵染引起了寄主基因表达的改变，这种改变是其他环境胁迫导致的共同结果。本研究发现，感染烟草花叶病毒（TRV）的拟南芥植物转录组反应与植物经历衰老的转录组反应之间存在着大量的重叠。这体现在病毒侵染中一些衰老相关暗诱导基因的上调，包括AtDIN1 (Senescence 1，SEN1)、AtDIN6 (Asparagine synthetase 1，AtASN1) 和 AtDIN11， 烟草中的DIN1、DIN6和DIN11同系物也被激活以响应TRV和马铃薯X病毒（PVX）的侵染。研究发现，DIN6和DIN11在植物防御反应激活前调节病毒的增殖，所以作者推测DIN6和DIN11在病毒侵染中发挥着作用。
外文摘要：Virus infections in plants cause changes in host gene expression that are common to other environmental stresses. In this work, we found extensive overlap in the transcriptional responses between Arabidopsis thaliana plants infected with Tobacco rattle virus (TRV) and plants undergoing senescence. This is exemplified by the up-regulation during infection of several senescence-associated Dark-inducible (DIN) genes, including AtDIN1 (Senescence 1, SEN1), AtDIN6 (Asparagine synthetase 1, AtASN1) and AtDIN11, DIN1, DIN6 and DIN11 homologues were also activated in Nicotiana benthamiana in response to TRV and Potato virus X (PVX) infection. Reduced TRV levels in RNA interference (RNAi) lines targeting AtDIN11 indicate that DIN11 is an important modulator of susceptibility to TRV in Arabidopsis. Furthermore, low accumulation of TRV in Arabidopsis protoplasts from RNAi lines suggests that AtDIN11 supports virus multiplication in this species. The effect of DIN6 on virus accumulation was negligible in Arabidopsis, perhaps as a result of gene or functional redundancy. However, TRV-induced silencing of NbASN, the DIN6 homologue in N. benthamiana, compromises TRV and PVX accumulation in systemically infected leaves. Interestingly, NbASN inactivation correlates with the appearance of morphological defects in infected leaves. We found that DIN6 and DIN11 regulate virus multiplication in a step prior to the activation of plant defence responses. We hypothesize on the possible roles of DIN6 and DIN11 during virus infection.
 
外文关键词：Arabidopsis thaliana; Dark-inducible (DIN) genes; Nicotiana benthamiana; plant viruses; Potato virus X; senescence-associated genes; Tobacco rattle virus
作者：Fernandez-Calvino, Lourdes; Guzman-Benito, Irene; Del Toro, Francisco J.; 等
作者单位：西班牙高等科研理事会
期刊名称：MOLECULAR PLANT PATHOLOGY
期刊影响因子：4.335
出版年份：2016
出版刊次：1
点击下载：在感染过程中激活衰老相关的暗诱导（DIN）基因有助于增强对植物病毒的敏感性