中文摘要：在中国，番茄黄化曲叶病毒（TYLCV）是由烟粉虱MEAM1传播的。玫烟色棒束孢是烟粉虱真菌病原体。然而，真菌感染对TYLCV的表达和MEAM1传播的影响仍是未知。本文将含有MEAM1二龄若虫的盆栽番茄经玫烟色棒束孢IfB01胁迫处理后，研究了MEAM1真菌感染与MEAM1传播TYLCV能力之间的关系。研究结果表明：玫烟色棒束孢感染降低了MEAM1中TYLCV的积累并减少了TYLCV的传播。本研究为寄主植物、植物病毒、昆虫介体和昆虫病原真菌之间的关系提供了新的见解。
外文摘要：Tomato yellow leaf curl virus (TYLCV) is transmitted by the Bemisia tabaci pest Middle East-Asia Minor 1 (MEAM1) in China. Isaria fumosorosea is a fungal pathogen of B. tabaci. However, the effects of fungal infection on TYLCV expression and transmission by MEAM1 are unclear. In this study, potted tomatoes containing second instar nymphs of MEAM1 were treated with I. fumosorosea IfB01 strain and the relationship between fungal infection in MEAM1 and its TYLCV transmission capacity was investigated. The results indicated that a significantly (p < 0.05) decreased incidence of transmission of TYLCV-infected plants (ITYPs) transmitted by second instar nymphs of MEAM1 infected with fungus. Further, we found a negative correlation between fungal conidial concentrations and eclosion rates of MEAM1, and a positive correlation between ITYPs and eclosion. In addition, when each plant was exposed to three adults treated with fungus, a significantly decreased transmission of TYLCV (TYTE) was observed in the infected group. However, the incidence of TYLCV-carrying MEAM1 adults (ITYAs) was not significantly different in the infected and control groups (p < 0.05). Nevertheless, a significant decrease in viral accumulation using TYLCV AC2 gene as a marker was observed in the fungus-infected MEAM1. In conclusion, the results suggested that I. fumosorosea infection decreases TYLCV accumulation in MEAM1 and subsequently reduces its transmission. Our study provides new insights into the relationship between host plant, plant virus, insect vector, and entomopathogenic fungus.
作者：Zhang, Bowen; Zou, Chunhua; Hu, Qiongbo
作者单位：华南农业大学
期刊名称：PLOS ONE
期刊影响因子：3.057
出版年份：2016
出版刊次：10
点击下载：玫烟色棒束孢对TYLCV（番茄黄化曲叶病毒）的积累和烟粉虱传播病毒能力的影响