中文摘要：烟粉虱是世界上最具破坏性的100个外来入侵物种之一。作为最重要的作物害虫和病毒介体之一，烟粉虱可导致大量作物减产，对全球食品安全构成了严重的威胁。本文报道了615-Mb的烟粉虱MEAM1隐种高通量基因组序列（粉虱科第一个基因组序列），该序列包含15664个蛋白编码基因。烟粉虱基因组包含了很多新奇的基因，其中包括与杀虫剂抗性、解毒作用和病毒传播有关的基因家族的扩展，而且还包括很多从细菌与真菌水平转移的基因。这些新奇的基因很可能促使烟粉虱变成具有高入侵性的杂食性作物害虫以及植物病毒的有效介体。这些基因组给解析烟粉虱隐种复合种群、理解基本的新奇生物产物提供了参考，并为控制烟粉虱及其传播病毒的新策略的制定提供了有价值的信息。
外文摘要：Background: The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is among the 100 worst invasive species in the world. As one of the most important crop pests and virus vectors, B. tabaci causes substantial crop losses and poses a serious threat to global food security. Results: We report the 615-Mb high-quality genome sequence of B. tabaci Middle East-Asia Minor 1 (MEAM1), the first genome sequence in the Aleyrodidae family, which contains 15,664 protein-coding genes. The B. tabaci genome is highly divergent from other sequenced hemipteran genomes, sharing no detectable synteny. A number of known detoxification gene families, including cytochrome P450s and UDP-glucuronosyltransferases, are significantly expanded in B. tabaci. Other expanded gene families, including cathepsins, large clusters of tandemly duplicated B. tabaci-specific genes, and phosphatidylethanolamine-binding proteins (PEBPs), were found to be associated with virus acquisition and transmission and/or insecticide resistance, likely contributing to the global invasiveness and efficient virus transmission capacity of B. tabaci. The presence of 142 horizontally transferred genes from bacteria or fungi in the B. tabaci genome, including genes encoding hopanoid/sterol synthesis and xenobiotic detoxification enzymes that are not present in other insects, offers novel insights into the unique biological adaptations of this insect such as polyphagy and insecticide resistance. Interestingly, two adjacent bacterial pantothenate biosynthesis genes, panB and panC, have been co-transferred into B. tabaci and fused into a single gene that has acquired introns during its evolution. Conclusions: The B. tabaci genome contains numerous genetic novelties, including expansions in gene families associated with insecticide resistance, detoxification and virus transmission, as well as numerous horizontally transferred genes from bacteria and fungi. We believe these novelties likely have shaped B. tabaci as a highly invasive polyphagous crop pest and efficient vector of plant viruses. The genome serves as a reference for resolving the B. tabaci cryptic species complex, understanding fundamental biological novelties, and providing valuable genetic information to assist the development of novel strategies for controlling whiteflies and the viruses they transmit.
外文关键词：Whitefly; Bemisia tabaci; Draft genome; Virus transmission; Polyphagy; Insecticide resistance
作者：Chen, Wenbo; Hasegawa, Daniel K.; Kaur, Navneet; 等
作者单位：美国康奈尔大学
期刊名称：BMC BIOLOGY
期刊影响因子：6.967
出版年份：2016
出版刊次：12
点击下载：全球作物害虫烟粉虱MEAM1隐种的基因组图为病毒传播、寄主适应性和杀虫剂抗性提供了新的视角