中文摘要：水稻齿叶矮缩病毒（RRSV）仅由烟粉虱以持久增殖型方式传播。已知RNA病毒性扩散与昆虫寄主细胞的细胞内膜有关。然而，RRSV增殖与细胞内膜之间互作的分子机制仍然未知。确定RRSV蛋白是否直接与其寄主细胞的细胞内膜成分互作将有很大的意义。本研究利用酵母双杂交系统鉴定了一个与寄主寡霉素敏感授予蛋白（OSCP）交互作用的RRSV非结构蛋白Pns10。通过GST pull-down分析法证实RRSV Pns10和褐飞虱OSCP间的交互作用。这些研究结果揭示了以寄主线粒体膜为靶点的病毒蛋白的新方面，并为关于病毒在昆虫介体内以线粒体膜蛋白为基础的增殖模式提供了新见解。
外文摘要：Rice ragged stunt virus (RRSV; Reoviridae) is exclusively transmitted by the brown planthopper Nilaparvata lugens in a persistent-propagative manner. It is understood that RNA viral proliferation is associated with the intracellular membranes of the insect host cells. However, the molecular mechanisms of the interaction between the RRSV proliferation and the intracellular membranes remain essentially unknown. It will be of great interest to determine whether RRSV protein(s) directly interact with intracellular membrane components of its host cells. In this study, we identified a RRSV nonstructural protein Pns10 interacting with a host oligomycin-sensitivity conferral protein (OSCP) using yeast two-hybrid system. The interaction between RRSV Pns10 and N. lugens OSCP was verified by a glutathione S-transferase pull-down assay. Confocal miscopy revealed colocalization of these two proteins in the cytoplasm of the salivary gland cells during the viral infection. The virions were further detected in the mitochondria under confocal miscopy and transmission electron microscopy combined with western blotting assay. This is the first observation that RRSV protein has a direct link with mitochondria. Suppressing OSCP gene expression by RNA interference notably decreased the viral loads in RRSV-infected insects. These findings revealed novel aspects of a viral protein in targeting the host mitochondrial membrane and provide insights concerning the mitochondrial membrane protein-based virus proliferation mode in the insect vector.
外文关键词：Oligomycin-sensitivity conferral protein; Rice ragged stunt virus; Pns10; Nilaparvata lugens; Virus-host protein interaction
作者：Huang, Hai-Jian; Liu, Cheng-Wen; Zhou, Xiang; 等
作者单位：浙江大学
期刊名称：VIRUS RESEARCH
期刊影响因子：2.526
出版年份：2017
出版刊次：2
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