中文摘要：在过去的几十年中，番茄黄化曲叶病毒（TYLCD）已经导致了热带和亚热带地区严重减产，发生率增长。在阿曼发现了六种与TYLCD有关的不同的菜豆金色花叶病毒属病毒。然而，阿曼番茄黄化曲叶病毒（TYLCV-OM）是全国分布范围最广的种类。本文研究开发了一种用于TYLCV-OM的带有内部控制（番茄延伸因子1（EF1））的实时定量PCR（qPCR）检测法，该检测法基于SYBRGreen化学，可对病毒进行快速检测并定量。
外文摘要：During the last few decades, Tomato yellow leaf curl disease (TYLCD) has resulted in heavy yield losses and increased incidences in tropical and subtropical regions. In Oman, six different species of begomovimses were found to be associated with TYLCD. However, Tomato yellow leaf curl virus- Oman (TYLCV-OM) was found to be the most widely distributed species in the whole country. A real-time quantitative PCR (qPCR) assay with an internal control [tomato elongation factor 1(EF1)] was developed for TYLCV-OM, based on SYBRGreen chemistry for the rapid detection and quantification of the virus. This assay was carried out on field samples showing symptoms according to The World Vegetable Center (AVRDC) disease severity scales. The test was used to establish a relationship between virus load and phenotypic symptoms. Viral copies of 2.88 x 10(9) were detected in field infected tomato plants showing symptom severity according to AVRDC scale '3' (i.e. severe leaf curling and yellowing of leaves). The copy number of virus decreased with the relative decrease in symptom severity scale. Tomato plants exhibiting scale '1' and '2' have 7.7 x 10(4) and 7.47 x 10(6) viral copies, respectively. Very low copy number of the virus (564) was detected in tomato plants showing symptoms at a severity scale of '0', which were apparently symptomless. However, tomato plants developed by tissue culture in sterilized conditions, which were used as a negative control, did not show the presence of the virus. The developed qPCR assay could detect as low as 18 fg (femto gram) of virus from total nucleic acid equivalent to approximately 30 genomic units. This quantitative assay can be used to determine virus titer, in breeding programs for development of virus resistant plants and for epidemiological surveys to monitor viral populations and their intensities.
外文关键词：TYLCV; Lycopersicon esculentum
作者：Ammara, Um E.; Al-Sadi, Abdullah Mohammed; Al-Shihi, Add; 等
作者单位：卡布斯苏丹大学
期刊名称： INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY
期刊影响因子：0.758
出版年份：2017
出版刊次：2
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