菜豆金色花叶病毒是植物病毒的主要群体，仅仅通过烟粉虱以持续的、非增殖型循环方式传播。目前，关于菜豆金色花叶病毒与粉虱互作的分子细胞机制研究很少。已有研究表明，昆虫肠道中有一些重要的蛋白受体，可以使病毒进入昆虫的血淋巴内。本研究以新德里番茄曲叶病毒（ToLCV）和拉贾斯坦棉花曲叶病毒（CLCuV）的外壳蛋白做诱饵，利用酵母双杂交技术筛选了肠道 cDNA 表达文库。研究结果表明，中肠蛋白（MGP）是一种可与ToLCV和CLCuV互作的常见蛋白。用抗中肠蛋白抗体喂养烟粉虱发现，ToLCV传播率下降了70%，这表明中肠蛋白（MGP）在病毒传播中发挥着积极的作用。
外文摘要：
Begomoviruses are a major group of plant viruses, transmitted exclusively by Bemisia tabaci (Gennadius) in a persistent circulative non-propagative manner. The information regarding molecular and cellular basis underlying Begomovirus - whitefly interaction is very scarce. Evidences have suggested that the insect gut possesses some crucial protein receptors that allow specific entry of virus into the insect haemolymph. We have performed yeast two hybrid gut cDNA expression library screening against coat protein of Tomato leaf curl New Delhi virus (ToLCV) and Cotton leaf curl Rajasthan virus (CLCuV) as bait. Midgut protein (MGP) was the common protein found interacting with both ToLCV and CLCuV. MGP was localized in whole mount B.tabaci as well as in dissected guts through confocal microscopy. Pull down and dot blot assays confirmed in vitro interaction between ToLCV/CLCuV coat protein and MGP. Immunolocalization analysis also showed colocalization of ToLCV/CLCuV particles and MGP within insect's gut. Finally, anti-MGP antibody fed B.tabaci, exhibited 70% reduction in ToLCV transmission, suggesting a supportive role for MGP in virus transmission.
外文关键词：B. tabaci；Begomovirus；receptor
作者：Rana, Vipin Singh；Popli, Sonam；Saurav, Gunjan Kumar；等
作者单位：德里大学
期刊名称：CELLULAR MICROBIOLOGY
期刊影响因子：4.46
出版年份：2016
出版刊次：5
点击下载：烟粉虱中肠蛋白与菜豆金色花叶病毒属（begomoviruses）之间的互作及其在病毒传播中发挥的作用研究