植物与植食性昆虫之间的互作非常复杂。水稻与褐飞虱互作是研究植物-昆虫互作的典范。本文开展了水稻与褐飞虱的代谢组学分析,旨在研究寄主水稻的防御机制以及褐飞虱的响应机制。研究检测了水稻叶鞘提取物中的26种代谢物,代谢组学分析结果显示,取食水稻的褐飞虱引起YHY15和TN1植株的代谢谱发生明显变化。本文开展的代谢组学研究对理解植物与植食性昆虫之间的互作有重要的意义,并为研究新的褐飞虱治理策略提供了必要的依据。
外文摘要:
Introduction:The interactions between plants and insect herbivores are complex and multifaceted. Rice and its specialist insect pest the brown planthopper (BPH), Nilaparvata lugens Stal (Hemiptera: Delphacidae) constitute an ideal system for studying plant-insect interactions.
Objectives:Combined metabolomics analyses of rice plant and BPH were conducted to understand the mechanism of host rice plant defense and BPH insect response.
Methods:Metabolite dynamics in rice leaf sheath and BPH honeydew was investigated using the gas chromatography-mass spectrometry (GC-MS) method. The GC-MS data were analyzed by principal component analysis and partial least squares-discriminant analysis.
Results:Twenty-six metabolites were detected in the leaf sheath extracts. Rice leaf sheath metabolomics analysis results show that BPH feeding induces distinct changes in the metabolite profiles of YHY15 and TN1 plants. These results suggest that BPH infestation enhance fatty acid oxidation, the glyoxylate cycle, gluconeogenesis and the GABA shunt in TN1 plants, and glycolysis and the shikimate pathway in YHY15. We propose that the BPH15 gene mediates a resistance reaction that increases the synthesis of secondary metabolites through the shikimate pathway. Thirty-three metabolites were identified in BPH honeydew. Honeydew metabolomics analysis results show that when BPH insects were fed on resistant YHY15 plants, most of the amino acids in honeydew were significantly decreased compared to those of BPH fed on TN1 plants. Based on metabolomics results, we propose that BPH feeding on resistant YHY15 plants would enhance amino acid absorption. At the same time, urea was significantly increased in BPH fed on YHY15.
Conclusion:Metabolomics study is valuable in understanding the complex and multifaceted interaction between plants and insect herbivores and provide essential clue for development of novel control BPH strategies.
外文关键词:Rice (Oryza sativa L.) plants;BPH15;Brown planthopper;Biotype;Metabolomics;GC–MS
作者:Lei Peng;Yan Zhao;Huiying Wang;Jiajiao Zhang;Chengpan Song;Xinxin Shangguan;Lili Zhu;Guangcun He;等
作者单位:武汉大学
期刊名称:Metabolomics
期刊影响因子:3.661
出版年份:2016
出版刊次:8
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