中文摘要：节肢动物唐氏综合征细胞黏附分子（Dscam）通过由选择性剪接产生的大量蛋白亚型介导病原菌-特异性识别。迄今为止，大多数研究集中在病原体-特异性免疫反应上，而病毒或内共生体进入细胞方面的研究较少。本研究克隆并标记了灰飞虱细胞黏附分子cDNA的特征，研究了LsDscam在水稻条纹叶枯病毒侵染过程中的功能以及对内共生体沃尔巴克氏体的影响。研究结果表明，特定的Dscam亚型可能会增强介体传播的病毒或内共生体的侵染。
外文摘要：The arthropod Down syndrome cell adhesion molecule (Dscam) mediates pathogen-specific recognition via an extensive protein isoform repertoire produced by alternative splicing. To date, most studies have focused on the subsequent pathogen-specific immune response, and few have investigated the entry into cells of viruses or endosymbionts. In the present study, we cloned and characterized the cDNA of Laodelphax striatellus Dscam (LsDscam) and investigated the function of LsDscam in rice stripe virus (RSV) infection and the influence on the endosymbiont Wolbachia. LsDscam displayed a typical Dscam domain architecture, including 10 immunoglobulin (Ig) domains, six fibronectin type III domains, one transmembrane domain and a cytoplasmic tail. Alternative splicing occurred at the N-termini of the Ig2 and Ig3 domains, the complete Ig7 domain, the transmembrane domain and the C-terminus, comprising 10, 51, 35, two and two variable exons, respectively. Potentially LsDscam could encode at least 71 400 unique isoforms and 17 850 types of extracellular regions. LsDscam was expressed in various L. striatellus tissues. Knockdown of LsDscam mRNA via RNA interference decreased the titres of both RSV and Wolbachia, but did not change the numbers of the extracellular symbiotic bacterium Acinetobacter rhizosphaerae. Specific Dscam isoforms may play roles in enhancing the infection of vector-borne viruses or endosymbionts.
外文关键词：Down syndrome cell adhesion molecule；Laodelphax striatellus；rice stripe virus infection
作者：Zhang, F.；Li, Q.；Chen, X.；等
作者单位：中国科学院
期刊名称：INSECT MOLECULAR BIOLOGY
期刊影响因子：2.866
出版年份：2016
出版刊次：8
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