中文摘要：白背飞虱因其取食具有破坏性，是水稻上较严重的害虫之一。白背飞虱唾液腺在其取食行为中起着重要的作用。然而，目前对于唾液腺分子水平上的认知非常少。本研究采用测序技术对白背飞虱成虫的唾液腺转录组进行了测序。研究确定了来自白背飞虱转录组的32种唾液蛋白基因，这些基因按照参与糖类代谢、脱毒、抑制植物防御反应、免疫相关反应、常规消化及其他植食性过程进行分类。本研究开展的白背飞虱唾液腺转录组分析研究，确定了参与取食过程的基因。本研究获得的数据对白背飞虱与寄主植物之间的互作机制研究有着重要的意义。
外文摘要：The white-backed planthopper (WBPH), Sogatella furcifera (Horvath), is one of the serious rice pests because of its destructive feeding. The salivary glands of the WBPH play an important role in the feeding behaviour. Currently, however, very little is known about the salivary glands at the molecular level. We sequenced the salivary gland transcriptome (sialotranscripome) of adult WBPHs using the Illumina sequencing. A total of 65,595 transcripts and 51,842 unigenes were obtained from salivary glands. According to annotations against the Nr database, many of the unigenes identified were associated with the most studied enzymes in hemipteran saliva. In the present study, we identified 32 salivary protein genes from the WBPH sialotranscripome, which were categorized as those involved in sugar metabolism, detoxification, suppression of plant defense responses, immunity-related responses, general digestion, and other phytophagy processes. Tissue expression profiles analysis revealed that four of 32 salivary protein genes (multicopper oxidase 4, multicopper oxidase 6, carboxylesterase and uridine phosphorylase 1 isform X2) were primarily expressed in the salivary gland, suggesting that they played putative role in insect-rice interactions. 13 of 32 salivary protein genes were primarily expressed in gut, which might play putative role in digestive and detoxify mechanism. Development expression profiles analysis revealed that the expression level of 26 of 32 salivary protein genes had no significant difference, suggesting that they may play roles in every developmental stages of salivary gland of WBPH. The other six genes have a high expression level in the salivary gland of adult. 31 of 32 genes (except putative acetylcholinesterase 1) have no significant difference in male and female adult, suggesting that their expression level have no difference between sexes. This report analysis of the sialotranscripome for the WBPH, and the transcriptome provides a foundational list of the genes involved in feeding. Our data will be useful to investigate the mechanisms of interaction between the WBPH and the host plant.
作者：Li, Zhen；An, Xing-Kui；Liu, Yu-Di；等
作者单位：中国农业科学院
期刊名称：PLOS ONE
期刊影响因子：3.057
出版年份：2016
出版刊次：7
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