中文摘要：水稻条纹叶枯病毒（RSV）和水稻黑条矮缩病毒（RBSDV）的侵染可使粮食生产发生重大损失。由于缺乏针对这些病毒的自然抗性资源，急需寻找一种新的生物技术方法，以提供有效且安全的RSV和RBSDV免疫力。本研究结果表明，抗性水平和amiRNAs相关；amiRNAs连同次级siRNAs一起，可介导对病毒RNAs的降解。遗传稳定性试验表明，转基因和amiRNA介导的病毒抗性可在转基因植物中稳定遗传。
外文摘要：Infection of rice with Rice stripe virus (RSV) and Rice black streaked dwarf virus (RBSDV) causes a significant loss of grain production. Due to the lack of natural resistance resources against these viruses, it is imperative to discover a biotechnological approach that will provide effective and safe immunity to RSV and RBSDV. In this study, we constructed three dimeric artificial microRNA (amiRNA) precursor expression vectors (pamiR-M, pamiR-3 and pamiR-U) that simultaneously target the CP genes of RSV and RBSDV based on the structure of the rice (Oryza sativa L.) osa-MIR528 precursor. The transgenic plants were obtained by Agrobacterium tumefaciens-mediated transformation and were shown to express amiRNAs successfully. Viral challenge assays revealed that these transgenic plants demonstrated different resistance (26.66-54.17 %) against RSV and RBSDV infection simultaneously. The amiRNA-targeting 3'-UTR region of CP gene (pamiR-U) induced higher virus resistance: 54.17 % against RSV and 45.83 % against RBSDV. A northern blot assay indicated that there was a good correlation between the resistance level and amiRNAs accumulation. The RNA silencing induced by the original amiRNAs could be bilaterally extended by the siRNA pathway. The amiRNAs, together with the secondary siRNAs, mediated the degradation of viral RNAs. A genetic stability assay showed that transgenes and amiRNA-mediated virus resistance could be stably inherited in the transgenic plants.
外文关键词：Artificial；microRNAs；Rice stripe virus；Rice black streaked dwarf virus；Transgenic rice；Multiple virus resistances
作者：Sun, Lin；Lin, Chao；Du, Jinwen；等
作者单位：山东农业大学
期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE
期刊影响因子：2.39
出版年份：2016
出版刊次：7
点击下载：二元人工microRNAs介导的高抗RSV和RBSDV转基因水稻研究