中文摘要：RNA干扰（RNAi）是一种有效的基因沉默工具，双链RNA（dsRNA）是一种研究昆虫基因功能的有效策略。本文目的在于研究从褐飞虱（水稻上典型的刺吸式口器昆虫）中分离出来的海藻糖酶基因（TRE 1-1、TRE 1-2和 TRE-2）的功能，并研究这些海藻糖酶基因通过dsRNA导入到幼虫中后对几丁质合成的调节作用。研究结果显示，TRE1和TRE2表现出互补关系，一方沉默时另一方的表达就会增强。本研究结果表明，TREs表达抑制剂可能会成为有效控制稻飞虱的工具。
 
外文摘要：RNA interference (RNAi) is an effective gene-silencing tool, and double stranded RNA (dsRNA) is considered a powerful strategy for gene function studies in insects. In the present study, we aimed to investigate the function of trehalase (TRE) genes (TRE 1-1, TRE 1-2, and TRE-2) isolated from the brown planthopper Nilaparvata lugens, a typical piercing-sucking insect in rice, and investigate their regulating roles in chitin synthesis by injecting larvae with dsRNA. The results showed that TRE1 and TRE2 had compensatory function, and the expression of each increased when the other was silenced. The total rate of insects with phenotypic deformities ranged from 19.83 to 24.36% after dsTRE injection, whereas the mortality rate ranged from 14.16 to 31.78%. The mRNA levels of genes involved in the chitin metabolism pathway in RNA-Seq and DGEP, namely hexokinase (HK), glucose-6-phosphate isomerase (G6PI) and chitinase (Cht), decreased significantly at 72 h after single dsTREs injection, whereas two transcripts of chitin synthase (CHS) genes decreased at 72 h after dsTRE1-1 and dsTREs injection. These results demonstrated that TRE silencing could affect the regulation of chitin biosynthesis and degradation, causing moulting deformities. Therefore, expression inhibitors of TREs might be effective tools for the control of planthoppers in rice.
作者：Zhao, Lina；Yang, Mengmeng；Shen, Qida；等
作者单位：杭州师范大学
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：5.228
出版年份：2016
出版刊次：6
点击下载：通过RNA干扰调节水稻褐飞虱内几丁质代谢路径的三种海藻糖酶基因功能特征