中文摘要：与健康的灰飞虱（SBPH）雌虫相比，感染了水稻条纹叶枯病毒（RSV）的灰飞虱雌虫通常产卵量较少，并且孵化周期长、孵化率低，容易引起畸形、智障或缺陷发育。本文采用同位素标记相对和绝对定量（iTRAQ）技术分析了带毒灰飞虱雌虫和健康灰飞虱雌虫卵细胞的差异蛋白质组学性能，以探索上述现象的分子机制。研究结果显示，与带毒卵细胞减数分裂和有丝分裂相关的差异性蛋白质表达可能会导致孵化率低、发育缺陷或迟缓等现象。参与呼吸链与营养代谢的蛋白质丰度的改变，可能会影响胚胎发育。本研究结果解释了宏观的发育现象，并探索了水稻条纹叶枯病毒对SBPH发育的影响机制。
外文摘要：Rice stripe virus-infected females of the small brown planthopper (SBPH, Laodelphax striatellus) usually lay fewer eggs with a longer hatch period, low hatchability, malformation and retarded or defective development compared with healthy females. To explore the molecular mechanism of those phenomena, we analyzed the differential proteomics profiling of the ova between viruliferous and healthy female insects using an isobaric tag for relative and absolute quantitation (iTRAQ) approach. We obtained 147 differentially accumulated proteins: 98 (66.7%) proteins increased, but 49 (33.3%) decreased in the ova of the viruliferous females. RT-qPCR was used to verify the 12 differential expressed proteins from iTRAQ, finding that trends in the transcriptional change for the 12 genes were consistent with those at the proteomic level. Differentially expressed proteins that were associated with meiosis (serine/threonine-protein phosphatase 2B and cyclin B3) and mitosis (cyclin B3 and dynein heavy chain) in viruliferous ova may contribute to low hatchability and defective or retarded development. Alterations in the abundance of proteins involved in the respiratory chain and nutrition metabolism may affect embryonic development. Our study begins to explain macroscopical developmental phenomena and explore the mechanisms by which Rice stripe virus impacts the development of SBPH.
作者：Liu, Beibei；Qin, Faliang；Liu, Wenwen；等
作者单位：中国农业科学院
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：5.228
出版年份：2016
出版刊次：6
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