中文摘要：WRKY转录因子不仅在植物生长发育中发挥着核心作用，在植物应激反应中也发挥着重要作用。然而，目前对于WRKY转录因子在植食性昆虫诱导植物（尤其是水稻）抗性及其潜在的作用机制方面仍然未知。本研究克隆了一种水稻WRKY基因OsWRKY45，机械损伤、褐飞虱侵染及JA或SA处理可诱导该基因的表达。研究结果表明，OsWRKY45在水稻对病原菌和植食性昆虫的抗性中发挥着重要的调节作用，OsWRKY45是否可用于培育抗病或抗植食性昆虫的水稻品种还值得进一步研究。
外文摘要：WRKY transcription factors play a central role not only in plant growth and development but also in plant stress responses. However, the role of WRKY transcription factors in herbivore-induced plant defenses and their underlying mechanisms, especially in rice, remains largely unclear. Here, we cloned a rice WRKY gene OsWRKY45, whose expression was induced by mechanical wounding, by infestation of the brown planthopper (BPH, Nilaparvata lugens) and by treatment with jasmonic acid (JA) or salicylic acid (SA). The antisense expression of OsWRKY45 (as-wrky) enhanced BPH-induced levels of H2O2 and ethylene, reduced feeding and oviposition preference as well as the survival rate of BPH, and delayed the development of BPH nymphs. Consistently, lower population densities of BPH on as-wrky lines, compared to those on wild-type (WT) plants, were observed in field experiments. On the other hand, as-wrky lines in the field had lower susceptibility to sheath blight (caused by Rhizoctonia solani) but higher susceptibility to rice blast (caused by Magnaporthe oryzae) than did WT plants. These findings suggest that OsWRKY45 plays important but contrasting roles in regulating the resistance of rice to pathogens and herbivores, and attention should be paid if OsWRKY45 is used to develop disease or herbivore-resistant rice.
外文关键词：rice；WRKY transcription factor；OsWRKY45；Nilaparvata lugens；herbivore-induced plant defense；H2O2；ethylene
作者：Huangfu, Jiayi；Li, Jiancai；Li, Ran；等
作者单位：浙江大学
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
期刊影响因子：3.257
出版年份：2016
出版刊次：6
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