中文摘要：番茄黄化曲叶病毒（TYLCV）是一种菜豆金黄花叶病毒属病毒，经烟粉虱传播到番茄及其他作物上。TYLCV蛋白因寄主防御而存在危险。本研究分析了番茄及烟粉虱昆虫介体对六种由TYLCV编码的蛋白质的降解能力。研究结果表明，在含有可溶性蛋白质、较少量大型蛋白质聚集体的片段中，番茄和烟粉虱的蛋白水解活性最高；中等大小蛋白质聚集体中，TYLCV蛋白水解作用明显减弱。TYLCV可以通过选择隐藏在小型/中型聚集体（在小型/中型聚集体中，只有很少的病毒蛋白会被蛋白质水解）中来应对寄主造成的降解危机。本研究首次比较了植物和昆虫寄主对TYLCV蛋白的降解机制。
外文摘要：Tomato yellow leaf curl virus (TYLCV) is a begomovirus transmitted by the whitefly Bemisia tabaci to tomato and other crops. TYLCV proteins are endangered by the host defenses. We have analyzed the capacity of the tomato plant and of the whitefly insect vector to degrade the six proteins encoded by the TYLCV genome. Tomato and whitefly demonstrated the highest proteolytic activity in the fractions containing soluble proteins, less-in large protein aggregates; a significant decrease of TYLCV proteolysis was detected in the intermediate-sized aggregates. All the six TYLCV proteins were differently targeted by the cytoplasmic and nuclear degradation machineries (proteases, ubiquitin 26S proteasome, autophagy). TYLCV could confront host degradation by sheltering in small/midsized aggregates, where viral proteins are less exposed to proteolysis. Indeed, TYLCV proteins were localized in aggregates of various sizes in both host organisms. This is the first study comparing degradation machinery in plant and insect hosts targeting all TYLCV proteins.
外文关键词：Geminivirus；Protein degradation；Protein aggregation；Immuno-localization
作者：Gorovits, Rena；Fridman, Lilia；Kolot, Mikhail；等
作者单位：以色列希伯莱大学
期刊名称：VIRUS RESEARCH
期刊影响因子：2.526
出版年份：2016
出版刊次：2
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