中文摘要:烟粉虱至少由24种基因组组成,是一种危害比较严重的昆虫。除了基因组,他们的共生菌也影响着烟粉虱的生态学性质,如传播植物病毒的能力以及对杀虫剂的抵抗力等方面。因此,确定基因组及共生体的感染状况对虫害的有效控制有着重要的意义。本文研究了一种多重诊断方法,可明确地鉴定烟粉虱的3种入侵性基因组、1种土著基因组以及7种共生体。本方法可节约83%的实验时间与67%的实验成本,且与传统方法相比,具有以下特点:(1)使用了DNA粗提取样本;(2)多重PCR技术极大地减少了PCR所须的实验次数;(3)无需限制性内切酶酶解;(4)利用基于硼酸钠缓冲液的250 V电泳法得到分离良好的条带图谱仅需不到十分钟。
外文摘要:A serious pest insect Bemisia tabaci (Gennadius) consists of at least 24 genetic groups. In addition to the genetic groups, their symbiotic bacteria reportedly affect the ecological natures of B. tabaci such as the ability of these insects to transmit phytopathogenic viruses and resist insecticides. Thus, it is important to identify the genetic groups and the symbiont infection status for effective pest control. Here, we describe a multiplex diagnostic method that clearly identified three invasive and an indigenous genetic group (MEAM1, MED Q1, MED Q2 and Asia II 6) and seven symbionts (Portiera, Hamiltonella, Rickettsia, Cardinium, Wolbachia, Arsenophonus and Hemipteriphilus) of B. tabaci. Our methods enabled up to 83 % time saving and 67 % cost reduction, compared to the conventional methods, due to the following features: (1) crude DNA samples were used; (2) multiplex PCR greatly reduced the number of PCR experiments necessary; (3) restriction enzyme digestion was not required; (4) 250 V electrophoresis using sodium borate buffer yielded well-separated band patterns in only 10 min.
外文关键词:Bemisia tabaci;Genetic groups;Endosymbiotic bacteria;Multiplex diagnostic PCR
作者:Kurata, A.;Fujiwara, A.; Haruyama, N.;等
作者单位:Toyama Univ
期刊名称:APPLIED ENTOMOLOGY AND ZOOLOGY
期刊影响因子:1.006
出版年份:2016
出版刊次:2
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