中文摘要：RNA干扰可有效控制一些重要的经济性害虫。烟粉虱是一种严重的田间作物害虫，可在全球导致严重减产。先前已有研究表明，转基因植物介导RNAi可成功控制烟粉虱。但是，目前关注的焦点是无脱靶效应的靶标基因选择，因此，急需探索烟粉虱的特定靶标。本研究利用蛋白质组学方法挖掘烟粉虱RNAi特定靶标，并确定烟粉虱功能基因（n=11）特有的核苷酸序列。识别出的基因对于烟粉虱的生命周期至关重要，可将其导入到转基因作物中，从而有效控制田间烟粉虱。
外文摘要：RNA interference offers effective control of several economically important insect pests. Bemisia tabaci is an important field crop pest, which causes significant yield loss worldwide. In our earlier study, we have demonstrated successful control of B. tabaci through transgenic plant mediated RNAi. However, selection of target genes without off-target effect(s) has been major concern so far and therefore, a critical exploration for B. tabaci specific targets is frantically required. In this study, we have followed proteomics approach to discover B. tabaci specific targets for RNAi and identified unique nucleotide sequences in functional genes (n = 11) of the pest. For this, we have developed proteome profile of B. tabaci extract using two-dimensional electrophoresis. A total of 504 protein spots were analyzed on mass-spectrometer and 453 proteins including 246 non-redundant proteins have been identified successfully. Complementation of the proteome data with available nucleotide database has helped us to interpret the unique nucleotide sequences. These nucleotide stretches may serve as environmentally safe targets for RNAi mediated control of the pest through crop genetic engineering. To the best of our knowledge, it is the most complete proteome of any whitefly species. We have also demonstrated application of proteomics in the identification of functional transcripts for RNAi. 
Biological significance: Insects cause major loss to crop productivity through direct and indirect damages. Among them, hemipteran group of insects are major contributor of global crop yield loss. In current study, gel based proteome profile of B. tabaci (one of the major hemipteran crop insect pest) is developed and characterized, which is a gap area in field of whitefly biology. It is an important data set of future whitefly studies like insect-plant interaction, virulence of whiteflies, their control program and discovery of new pesticides. Out of various control strategies, RNA interference offers a great potential to combat the whitefly successfully. However, the uniqueness of target genes and off target impact of the technology remains a challenge to scientific community. We used our proteome data set for the identification of B. tabaci specific gene targets for RNAi mediated control. The identified genes are critical for the life cycle of B. tabaci hence, could be proven as good molecules for making transgenic crop plant for efficient control of whiteflies in the field.
外文关键词：B. tabaci；Proteome；RNAi；Whitefly；Ferritin
作者：Mishra, Manisha；Saurabh, Sharad；Maurya, Rashmi；等
作者单位：CSIR
期刊名称：JOURNAL OF PROTEOMICS
期刊影响因子：3.867
出版年份：2016
出版刊次：1
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