中文摘要：MicroRNAs (miRNAs)是一类新发现的内源性非编码小RNAs，在许多真核生物代谢活动中起着重要的调控作用。白背飞虱是唯一被确认的可传播南方水稻黑条矮缩病（SRBSDV）的昆虫介体，但关于白背飞虱miRNAs识别方面的研究报道较少。本研究通过基于带毒和不带毒白背飞虱两个小RNA文库的高通量测序，鉴定识别了382个miRNAs，包括106个保守的miRNA和276个新的miRNA，这些miRNA分别属于52个保守的miRNA家族与58个白背飞虱特有家族。本研究中对已识别的miRNAs的分析，有助于理解这些miRNAs在关于白背飞虱代谢、发育及病毒侵染的基因调控与表达中发挥的作用。
外文摘要：MicroRNAs (miRNAs) are a novel class of small, non-coding endogenous RNAs that play critical regulatory roles in many metabolic activities in eukaryotes. Reports of the identification of miRNAs in Sogatella furcifera (white-backed planthopper), the insect that acts as the only confirmed vector of the southern rice black-streaked dwarf virus (SRBSDV), are limited. In this study, a total of 382 miRNAs were identified in S. furcifera, including 106 conserved and 276 novel miRNAs, using high-throughput sequencing based on two small RNA libraries from viruliferous and non-viruliferous S. furcifera, and these miRNAs belonged to 52 conserved miRNA families and 58 S. furcifera-specific families, respectively. Comparison with miRNAs from 26 insect species and five other species in miRBase showed that more than half of the conserved miRNA families are highly conserved in Hexapoda, while othermiRNAs are only conserved in non-dipterans. Furthermore, 4 117 target genes predicted for the 382 identified miRNAs could be categorized into 45 functional groups annotated by Gene Ontology. Compared with non-viruliferous cells, eight up-regulated miRNAs and four down-regulated miRNAs were identified in cells inoculated with SRBSDV, among which miR-14 and miR-n98a may be involved in the immune response to SRBSDV infection. Analyses of the identified miRNAs will provide insights into the roles of these miRNAs in the regulation and expression of genes involved in the metabolism, development and viral infection of S. furcifera.
外文关键词：evolution；functional shift；genomic cluster；microRNA；Sogatella furcifera； SRBSDV
作者：Chang, Zhao-Xia；Tang, Nan；Wang, Lin；等
作者单位：中国科技大学
期刊名称：INSECT SCIENCE
期刊影响因子：2.551
出版年份：2016
出版刊次：6
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