中文摘要：水稻黑条矮缩病毒（RBSDV）隶属于呼肠孤病毒科斐济病毒属，并可导致亚洲水稻产区产量严重减少。RNA沉默作为抵抗植物病毒的一种天然防御机制，已成功应用于包括水稻在内的植物病毒抗性工程研究中。本研究发现的转基因水稻品系携带有发夹RNA（hpRNA）结构，该发夹RNA（hpRNA）结构以RBSDV基因S1、S2、S6和S10为靶标，分别编码RNA依赖的RNA聚合酶、核心蛋白、RNA沉默抑制子和外壳蛋白。本研究结果表明，以多个病毒基因为靶标的长hpRNA 可用于使水稻及其他植物产生稳定、持久的抗病毒性。
外文摘要：Rice black-streaked dwarf virus (RBSDV) belongs to the genus Fijivirus in the family of Reoviridae and causes severe yield loss in rice-producing areas in Asia. RNA silencing, as a natural defence mechanism against plant viruses, has been successfully exploited for engineering virus resistance in plants, including rice. In this study, we generated transgenic rice lines harbouring a hairpin RNA (hpRNA) construct targeting four RBSDV genes, S1, S2, S6 and S10, encoding the RNA-dependent RNA polymerase, the putative core protein, the RNA silencing suppressor and the outer capsid protein, respectively. Both field nursery and artificial inoculation assays of three generations of the transgenic lines showed that they had strong resistance to RBSDV infection. The RBSDV resistance in the segregating transgenic populations correlated perfectly with the presence of the hpRNA transgene. Furthermore, the hpRNA transgene was expressed in the highly resistant transgenic lines, giving rise to abundant levels of 21-24 nt small interfering RNA (siRNA). By small RNA deep sequencing, the RBSDV-resistant transgenic lines detected siRNAs from all four viral gene sequences in the hpRNA transgene, indicating that the whole chimeric fusion sequence can be efficiently processed by Dicer into siRNAs. Taken together, our results suggest that long hpRNA targeting multiple viral genes can be used to generate stable and durable virus resistance in rice, as well as other plant species.
外文关键词：RBSDV; hpRNA; RNAi; multiple viral genes; strong resistance
作者：Wang, Fangquan; Li, Wenqi; Zhu, Jinyan; 等
作者单位：江苏省农业科学院
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
期刊影响因子：3.257
出版年份：2016
出版刊次：5
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