中文摘要：持久性植物病毒通常依靠昆虫传播；它们不能在植物间或者通过机械接种传播。然而，对于持久性病毒在昆虫介体中的致病性机制仍然未知。本研究利用水稻条纹病毒（RSV）、病毒昆虫介体灰飞虱和寄主植物（水稻）来探讨持久性病毒如何从昆虫介体获得致病性。RSV可从灰飞虱的消化道和唾液腺获取植物病原体。本研究通过叶脉注射将RSV机械接种到水稻叶片上。研究结果发现，来自昆虫和来自植株的RSV在水稻上的表征不同，基因表达水平也不同；且来自昆虫的RSV损害了叶绿体内的基粒垛并抑制了光合作用。本研究不仅为机械接种RSV至植株上提供了一种更便捷的方法，也为研究来自昆虫介体和带毒植株的RSV的不同致病机理提供了一个新的视角。
外文摘要：Persistent plant viruses usually depend on insects for their transmission; they cannot be transmitted between plants or through mechanical inoculation. However, the mechanism by which persistent viruses become pathogenic in insect vectors remains unknown. In this study, we used Rice stripe virus (RSV), its insect vector Laodelphax striatellus and host plant (Oryza sativa) to explore how persistent viruses acquire pathogenicity from insect vectors.
RSV acquired phytopathogenicity in both the alimentary tract and the salivary gland of L. striatellus.
We mechanically inoculated RSV into rice O. sativa leaves through midrib microinjection. Insect-derived RSV induced a typical stripe symptom, whereas plant-derived RSV only produced chlorosis in rice leaves. Insect-derived RSV had higher expression of genes rdrp, ns2, nsvc2, sp and nsvc4 than plant-derived RSV, and the latter had higher expression of genes cp and ns3 than the former in rice leaves. Different from plant-derived RSV, insect-derived RSV damaged grana stacks within the chloroplast and inhibited photosynthesis by suppressing the photosystem II subunit psbp.
This study not only presented a convenient method to mechanically inoculate RSV into plants, but also provided insights into the different pathogenic mechanisms of RSV from the insect vector and from viruliferous plants.
 
外文关键词：alimentary canal；chloroplast; mechanical inoculation；rice stripe disease；salivary gland；small brown planthopper (Laodelphax striatellus)
作者：Zhao, Wan；Yang, Pengcheng;；Kang, Le；等
作者单位：中国科学院
期刊名称：NEW PHYTOLOGIST
期刊影响因子：7.21
出版年份：2016
出版刊次：4
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