中文摘要：很多小干扰RNA(siRNAs)是在植物病毒侵染过程中产生的，但其功能、特征与生物合成仍然未知。本研究采用高通量深度测序技术，分析了被黄瓜花叶病素（CMV）病毒Fny菌株侵染后的番茄植株病毒小RNA的情况。本研究结果表明由源自病毒的siRNAs的产生可能会参与病毒与寄主间的交互作用，且有助于解释病原菌是如何引起寄主植株的症状。
外文摘要：A Large number of short interfering RNAs (siRNAs) are generated from plant viruses during infection, but their function, character and biogenesis are unclear. Here, we analyzed the viral small RNA profile in tomato plants upon Cucumber mosaic virus (CMV) Fny strain infection, using high-throughput deep sequencing techniques. A set of 129666 CMV-Fny-specific small RNAs, most of the 21-(54.6%) and 22-nucleotide (28.2%) in size were obtained. Both strands of CMV-Fny were substrates for production of viral-specific siRNAs, and the virus genome harbored multiple, highly reproducible small RNA-generating hot spots that corresponded to regions with no apparent local hairpin-forming capacity. In addition, using the novel high throughput degradom sequencing approach and informatics analysis, we found a large set of host genes could be potentially targeted by CMV-Fny-derived siRNAs for posttranscriptional silencing. Six of such host targets were further validated by qRT-PCR. Gene ontology analysis with biological process, cellular component and molecular function terms revealed that these target genes mainly related to stress response, cell redox homeostasis, protein folding/stabilization/transport, chloroplast-and photosynthesis-related processes, metal ion binding, ATP binding, jasmonic acid mediated signaling pathway, and gene silencing by RNA. These results indicate that the production of viral derived siRNAs might be involved in virus-host interactions, and could contribute to explain how pathogens cause symptoms in their hosts.
外文关键词：Cucumber Mosaic Virus；Short Interfering RNA；Genome；High-Throughput Deep Sequencing；Degradom Analysis
作者：Feng, Junli；Liu, Shasha；Chen, Jishuang
作者单位：浙江工商大学
期刊名称：OURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
期刊影响因子：1.338
出版年份：2016
出版刊次：7
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