中文摘要：白背飞虱（WBPH）可刺吸植物汁液，并以循环、持久增殖型方式有效传播南方水稻黑条矮缩病毒（SRBSDV），现已成为水稻生产中最具破坏性的害虫之一。然而，对于动态且复杂的SRBSDV、WBPH与水稻间的交互作用仍知之甚少。本研究基于同源性全基因组分析，确定了隶属于28个家族的348个白背飞虱免疫相关基因。带毒和非带毒转录组分析表明，不管病毒滴度高低，昆虫取食被SRBSDV侵染过的水稻植株会对其基因表达产生很大的影响。本研究结果阐明了免疫基因和WBPH转录组对SRBSDV的响应，并提高了对病原体、介体昆虫与寄主植株之间功能关系的认知。
外文摘要：Sogatella furcifera, the white-backed planthopper (WBPH), has become one of the most destructive pests in rice production owing to its plant sap-sucking behavior and efficient transmission of Southern rice black-streaked dwarf virus (SRBSDV) in a circulative, propagative and persistent manner. The dynamic and complex SRBSDV-WBPH-rice plant interaction is still poorly understood. In this study, based on a homology-based genome-wide analysis, 348 immune-related genes belonging to 28 families were identified in WBPH. A transcriptome analysis of non-viruliferous (NVF) and viruliferous groups with high viral titers (HVT) and median viral titers (MVT) revealed that feeding on SRBSDV-infected rice plants has a significant impact on gene expression, regardless of viral titers in insects. We identified 278 up-regulated and 406 down-regulated genes shared among the NVF, MVT, and HVT groups and detected significant down-regulation of primary metabolism-related genes and oxidoreductase. In viruliferous WBPH with viral titer-specific transcriptome changes, 1,906 and 1,467 genes exhibited strict monotonically increasing and decreasing expression, respectively. The RNAi pathway was the major antiviral response to increasing viral titers among diverse immune responses. These results clarify the responses of immune genes and the transcriptome of WBPH to SRBSDV and improve our knowledge of the functional relationship between pathogen, vector, and host.
作者：Wang, Lin；Tang, Nan；Gao, Xinlei；等
作者单位：中国科技大学
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：5.228
出版年份：2016
出版刊次：11
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