中文摘要：水稻瘤矮病毒（RGDV）是一种植物呼肠孤病毒属病毒，威胁着东南亚及华南的水稻生产。RGDV由介体电光叶蝉以持久增殖性的方式传播。为了评估RGDV对电光叶蝉的直接影响，本研究通过采用水浸泡滤纸培养法培养带毒雌虫产下的卵，建立了一个感染病毒的叶蝉种群。通过本研究可揭示RGDV如何对电光叶蝉产生负面影响，这可阻止带毒昆虫种群的扩张。
外文摘要：Rice gall dwarf virus (RGDV), a plant reovirus that threatens rice production in Southeast Asia and Southern China, is transmitted by the leafhopper vector Recilia dorsalis in a persistent-propagative manner. To assess the direct effects of RGDV on R. dorsalis, we established an infected leafhopper population from eggs laid by viruliferous females using the water-soaked filter paper culture method. Life history parameters indicated that the virus was harmful to its vector in terms of all biotic indices, including reduced survival rate, emergence rate, fecundity, and longevity of adults, compared with a nonviruliferous control population. Those findings were supported by systematic monitoring of viruliferous rates of R. dorsalis in different overwintering generations. To better elucidate the adverse effects of RGDV on its vector, we measured fecundity at the molecular level using quantitative reverse transcription polymerase chain reaction and Western blot assays, which revealed differential expression of vitellogenin (Vg) in viruliferous versus nonviruliferous adult females. We infer that RGDV reduced levels of Vg transcript and protein product, resulting in the lower fecundity of its vector. Overall, this study demonstrates how RGDV exerts an adverse effect on R. dorsalis, which hinders the expansion of viruliferous populations of the insect.
作者：Chen, Yong；Lu, Chengcong；Li, Manman；等
作者单位：福建农林大学
期刊名称：PLANT DISEASE
期刊影响因子：3.192
出版年份：2016
出版刊次：4
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