中文摘要：目前，已成功采用抗虫转基因植物降低产量损失、减少农药使用。为了实现对指定转基因植物中咀嚼式和刺吸式害虫的共同治理，本研究研发了表达融合蛋白AaIT/GNA的转基因烟草、转基因拟南芥和转基因水稻。研究结果表明，转基因植物中AaIT/GNA融合蛋白的表达是控制害虫（尤其是Bt作物中对毒素不敏感的刺吸式害虫）的有效途径。
外文摘要：The adoption of pest-resistant transgenic plants to reduce yield losses and decrease pesticide use has been successful. To achieve the goal of controlling both chewing and sucking pests in a given transgenic plant, we generated transgenic tobacco, Arabidopsis, and rice plants expressing the fusion protein, AaIT/GNA, in which an insecticidal scorpion venom neurotoxin (Androctonus australis toxin, AaIT) is fused to snowdrop lectin (Galanthus nivalis agglutinin, GNA). Compared with transgenic tobacco and Arabidopsis plants expressing AaIT or GNA, transgenic plants expressing AaIT/GNA exhibited increased resistance and toxicity to one chewing pest, the cotton bollworm, Helicoverpa armigera. Transgenic tobacco and rice plants expressing AaIT/GNA showed increased resistance and toxicity to two sucking pests, the whitefly, Bemisia tabaci, and the rice brown planthopper, Nilaparvata lugens, respectively. Moreover, in the field, transgenic rice plants expressing AaIT/GNA exhibited a significant improvement in grain yield when infested with N. lugens. This study shows that expressing the AaIT/GNA fusion protein in transgenic plants can be a useful approach for controlling pests, particularly sucking pests which are not susceptible to the toxin in Bt crops.
外文关键词：AaIT；AaIT；GNA；chewing pest；GNA；fusion protein；pest control；resistance； sucking pest；toxicity；transgenic plant
作者：Liu, Shu-Min；Li, Jie；Zhu, Jin-Qi；等
作者单位：中国科学院上海生命科学院
期刊名称：INSECT SCIENCE
期刊影响因子：2.551
出版年份：2016
出版刊次：4
点击下载：表达AaIT/GNA融合蛋白的转基因植物可增强植物对咀嚼式和刺吸式害虫的抵抗力和毒力