中文摘要：褐飞虱是影响亚洲水稻生产的重要害虫，其爆发与农药诱导的生殖刺激紧密相关。因此，褐飞虱是一种典型的农药诱导再猖獗害虫。但是，褐飞虱的不同基因对农药诱导的生殖刺激的调节作用仍是未知。本文通过基因沉默研究了酰基辅酶A氧化酶（ACO）对褐飞虱生殖与生化方面的调节作用。研究结果表明，ACO是褐飞虱雌虫对三唑磷（TZP）诱导的生殖刺激起调节作用的关键基因。
外文摘要：The brown plant hopper (BPH), Nilaparvata lugens Stal (Hemiptera: Delphacidae), is a major pest affecting rice in Asia, and outbreaks of this pest are closely linked to pesticide-induced stimulation of reproduction. Therefore, the BPH is a classic example of a resurgent pest. However, the effects of different genes on the regulation of pesticide induced reproductive stimulation in the BPH are unclear. In this study, the regulatory effects of acyl-coenzyme A oxidase (ACO) on the reproduction and biochemistry of the BPH were investigated with gene silencing. The number of eggs laid per female by triazophos (TZP) + dsACO BPH females was significantly lower than those of TZP-treated (without ACO silencing) or TZP + GFP females (negative control), with the number of eggs decreasing by 30.8% (from 529.5 to 3663) and 32.0% (from 540.5 to 366.3), respectively. The preoviposition period, oviposition period, and longevity of the TZP-treated females were also influenced by dsACO treatment Additionally, the amounts of crude fat, protein, and some fatty acids (oleic acid, palmitic acid, linoleic acid, stearic acid, and myristoleic acid) in TZP + dsACO females were significantly lower than in TZP-treated females. Thus, ACO is one of the key genes regulating the TZP-induced stimulation of reproduction in BPH females.
外文关键词：Triazophos；Nilaparvata lugens；Reproduction；ACO gene；RNAi
作者：Liu, Zong-Yu；Jiang, Yi-Ping；Li, Lei；等
作者单位：扬州大学
期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
期刊影响因子：2.388
出版年份：2016
出版刊次：3
点击下载：ACO沉默可抑制三唑磷处理过的褐飞虱雌虫的生殖与能量代谢