中文摘要：水稻条纹病毒（RSV）可引起水稻条纹叶枯病，由灰飞虱（SBPH）以持久、循回增殖型方式传播。SBPH的消化道和唾液腺在病毒复制和传播中起着重要的作用。然而，对于这两种器官在RSV与SBPH间交互作用中的潜在分子作用仍知之甚少。本研究分析了带毒和不带毒SBPH的消化道与唾液腺器官的特异性转录组，研究结果为研究这两种器官在对抗RSV侵染中的作用提供了线索，并有助于理解RSV与SBPHs间的交互作用。
外文摘要：Rice stripe virus (RSV) causes rice stripe disease and is transmitted by the small brown planthopper (Laodelphax striatellus, SBPH) in a persistent, circulative, and propagative manner. The alimentary canal and salivary gland of SBPH play important roles in viral replication and transmission. However, little is known about the underlying molecular functions of these two organs in the interaction between RSV and SBPH. In this study, organ-specific transcriptomes of the alimentary canal and salivary gland were analyzed in viruliferous and naive SBPH. The number of differentially expressed unigenes in the alimentary canal was considerably greater than that in the salivary gland after RSV infection, and only 23 unigenes were co-regulated in the two organs. In the alimentary canal, genes involved in lysosome, digestion and detoxification were activated upon RSV infection, whereas the genes related to DNA replication and repair were suppressed. RSV activated RNA transport and repressed the MAPK, mTOR, Wnt, and TGF-beta signaling pathways in the salivary gland. The overall immune reaction toward RSV was much stronger in the salivary gland than in the alimentary canal. RSV activated the pattern recognition molecules and Toll pathway in the salivary gland but inhibited these two reactions in the alimentary canal. The responses from reactive oxygen and the immune-responsive effectors were stronger in the salivary gland than in the alimentary canal after RSV infection. These findings provide clues on the roles of the two organs in confronting RSV infection and aid in the understanding of the interaction between RSV and SBPHs.
外文关键词：Rice stripe virus；Small brown planthopper；Transcriptome；Salivary gland；Alimentary canal；Immune response
作者：Zhao, Wan；Lu, Lixia；Yang, Pengcheng；等
作者单位：中国科学院
期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
期刊影响因子：3.767
出版年份：2016
出版刊次：3
点击下载：灰飞虱对水稻条纹病毒的器官特异性转录反应研究