中文摘要：病毒诱导的基因沉默（VIGS）已经成为一种快速、高效筛选单子叶植物和双子叶植物功能基因组的新兴技术。已有研究认为大麦条纹花叶病毒（BSMV）是评估小麦和大麦病程相关基因的有效VIGS载体；然而，这些研究对于BSMV引起的表型和防御反应却知之甚少。通过本研究发现，使用减毒BSMV有利于小麦病程相关基因的描述；此外，减毒BSMV会引起防御激素积累的减少，这表明该基因型可应用于与植物激素相关的信号、发育反应和抗病性的调查研究中。
外文摘要：Background: Virus-induced gene silencing (VIGS) has become an emerging technology for the rapid, efficient functional genomic screening of monocot and dicot species. The barley stripe mosaic virus (BSMV) has been described as an effective VIGS vehicle for the evaluation of genes involved in wheat and barley phytopathogenesis; however, these studies have been obscured by BSMV-induced phenotypes and defense responses. The utility of BSMV VIGS may be improved using a BSMV genetic background which is more tolerable to the host plant especially upon secondary infection of highly aggressive, necrotrophic pathogens such as Fusarium graminearum.
Results: BSMV-induced VIGS in Triticum aestivum (bread wheat) cv. 'Fielder' was assessed for the study of wheat genes putatively related to Fusarium Head Blight (FHB), the necrotrophism of wheat and other cereals by F. graminearum. Due to the lack of 'Fielder' spike viability and increased accumulation of Fusarium-derived deoxynivalenol contamination upon co-infection of BSMV and FHB, an attenuated BSMV construct was generated by the addition of a glycinerich, C-terminal peptide to the BSMV gamma b protein. This attenuated BSMV effectively silenced target wheat genes while limiting disease severity, deoxynivalenol contamination, and yield loss upon Fusarium co-infection compared to the original BSMV construct. The attenuated BSMV-infected tissue exhibited reduced abscisic, jasmonic, and salicylic acid defense phytohormone accumulation upon secondary Fusarium infection. Finally, the attenuated BSMV was used to investigate the role of the salicylic acid-responsive pathogenesis-related 1 in response to FHB.
Conclusions: The use of an attenuated BSMV may be advantageous in characterizing wheat genes involved in phytopathogenesis, including Fusarium necrotrophism, where minimal viral background effects on defense are required. Additionally, the attenuated BSMV elicits reduced defense hormone accumulation, suggesting that this genotype may have applications for the investigation of phytohormone-related signaling, developmental responses, and pathogen defense.
外文关键词：Salicylic acid；Jasmonic acid；Abscisic acid；Pathogenesis-related 1； Deoxynivalenol； Vomitoxin；Yield；Necrotrophic fungi
作者：Buhrow, Leann M.；Clark, Shawn M.；Loewen, Michele C.
作者单位：Natl Res Council Canada
期刊名称：PLANT METHODS
期刊影响因子：3.449
出版年份：2016
出版刊次：2
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