中文摘要：植物呼肠孤病毒、弹状病毒、番茄斑萎病毒和纤细病毒属病毒由昆虫介体以持久、增殖型方式传播。但对于这些植物病毒如何在昆虫介体内建立并维持持续性感染仍知之甚少。本研究将植物病毒即水稻瘤矮病毒（RGDV）及其主要的昆虫介体电光叶蝉组成病毒-昆虫系统，来探究小干扰RNA（siRNA）通路是如何调节植物病毒在昆虫介体内持续性感染的。本研究结果揭示：昆虫介体内的小干扰RNA通路可调节植物病毒的持续性感染。
外文摘要：Plant reoviruses, rhabdoviruses, tospoviruses, and tenuiviruses are transmitted by insect vectors in a persistent-propagative manner. How such persistent infection of plant viruses in insect vectors is established and maintained remains poorly understood. In this study, we used rice gall dwarf virus (RGDV), a plant reovirus, and its main vector leafhopper Recilia dorsalis as a virus-insect system to determine how the small interference (siRNA) pathway modulates persistent infection of a plant virus in its insect vector. We showed that a conserved siRNA antiviral response was triggered by the persistent replication of RGDV in cultured leafhopper cells and in intact insects, by appearance of virusspecific siRNAs, primarily 21-nt long, and the increased expression of siRNA pathway core components Dicer-2 and Argonaute-2. Silencing of Dicer-2 using RNA interference strongly suppressed production of virus-specific siRNAs, promoted viral accumulation, and caused cytopathological changes in vitro and in vivo. When the viral accumulation level rose above a certain threshold of viral genome copy (1.32 x 10(14) copies/mu g insect RNA), the infection of the leafhopper by RGDV was lethal rather than persistent. Taken together, our results revealed a new finding that the siRNA pathway in insect vector can modulate persistent infection of plant viruses.
作者：Lan, Hanhong；Wang, Haitao；Chen, Qian；等
作者单位：福建农林大学
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：5.228
出版年份：2016
出版刊次：2
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