中文摘要：病毒感染可引起植株失绿黄化、矮缩、坏死或其他症状。叶绿体相关基因（ChRGs）的下调被认为是导致植株失绿黄化的原因。本研究在感染水稻条纹病毒（RSV）的烟草中确定了差异表达基因（DEGs），并通过烟草脆裂病毒（TRV）诱导的基因沉默使75个下调DEGs逐一沉默，且进一步调查了这75个下调DEGs对RSV症状的贡献。本研究结果为支持ChRGs与失绿黄化之间的相关性提供了实验证据，并表明eIF4A参与了RSV症状的发展。本研究首次报道了直接来自于某种植物病毒的siRNA可靶向调控寄主基因。
外文摘要：Viral infections cause plant chlorosis, stunting, necrosis or other symptoms. The down-regulation of chloroplast-related genes (ChRGs) is assumed to be responsible for chlorosis.We identified the differentially expressed genes (DEGs) in Rice stripe virus (RSV)-infected Nicotiana benthamiana, and examined the contribution of 75 down-regulated DEGs to RSV symptoms by silencing them one by one using Tobacco rattle virus (TRV)-induced gene silencing. Silencing of 11 of the 75 down-regulated DEGs caused plant chlorosis, and nine of the 11 were ChRGs. Silencing of a down-regulated DEG encoding the eukaryotic translation initiation factor 4A (eIF4A) caused leaf-twisting and stunting that were visible on RSV-infected N. benthamiana. A region of RSV RNA4 was complementary to part of eIF4A mRNA and virus-derived small interfering (vsiRNAs) from that region were present in infected N. benthamiana. When expressed as artificial microRNAs, those vsiRNAs could target NbeIF4A mRNA for regulation. We provide experimental evidence supporting the association of ChRGs with chlorosis and show that eIF4A is involved in RSV symptom development. This is also the first report demonstrating that siRNA derived directly from a plant virus can target a host gene for regulation.
外文关键词：chloroplast-related genes；chlorosis；eIF4A；Nicotiana benthamiana；Oryza sativa； Rice stripe virus (RSV)；symptom development；virus-derived siRNA
作者：Shi, Bingbin；Lin, Lin；Wang, Shihui；等
作者单位：浙江农业科学院
期刊名称：NEW PHYTOLOGIST
期刊影响因子：7.21
出版年份：2016
出版刊次：2
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