中文摘要：南方水稻黑条矮缩病毒（SRBSDV）是一种由白背飞虱传播的新型病毒。由于白背飞虱病毒传播效率低，研究者经常面临带毒载体或感染病毒的水稻植株较少的问题，尤其是在冬天和春天的时候。为了找到维持感染病毒的材料的新方法，本研究将染毒水稻植株在-80℃条件下贮藏45天或140天，以评估其能否作为昆虫介体传播病毒的病毒来源。研究结果表明，低温贮藏的感染SRBSDV的水稻植株不能维持白背飞虱对病毒的传播。
外文摘要：Southern rice black-streaked dwarf virus (SRBSDV) is a novel virus transmitted by white-backed planthopper Sogatella furcifera (Hovath) (Hemiptera: Delphacidae). Due to low virus transmission efficiency by the planthopper, researchers are frequently confronted with shortage of viruliferous vectors or infected rice plants, especially in winter and the following spring. To find new ways to maintain virus-infected materials, viral rice plants were stored at -80 degrees C for 45 or 140 d and evaluated as virus sources in virus transmission by the vector. SRBSDV virions were not degraded during storage at -80 degrees C as indicated by reverse transcription-polymerase chain reaction and reverse transcription real-time PCR detection. The planthopper nymphs fed on the infected thawed plants for 48 h survived at about 40% and showed positive detection of SRBSDV, but they lost the virus after feeding for another 20 d (the circulative transmission period) on noninfected plants. Transmission electron microscope images indicated broken capsid of virions in infected thawed leaves in contrast to integrity capsid of virions in infected fresh leaves. These results show that low temperature storage of SRBSDV-infected rice plants cannot sustain virus transmission by white-backed planthopper.
外文关键词：southern rice black-streaked dwarf virus；rice；white-backed planthopper；virus acquisition；low temperature storage
作者：Liu, Danfeng；Li, Pei；Han, Yongqiang；等
作者单位：中国农业科学院
期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY
期刊影响因子：1.609
出版年份：2016
出版刊次：2
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