中文摘要：番茄黄化曲叶病毒（TYLCV）是一种单链（ssDNA）菜豆金色花叶病毒属病毒，可对全球番茄和其他作物造成严重的损害。TYLCV仅由烟粉虱以持久、循环、增殖型方式传播。以往的研究表明，TYLCV在介体昆虫中的传播、存留与循环参与了昆虫与内共生菌蛋白质的交互作用，内共生菌蛋白质有助于病毒的传播，或对昆虫体内存在的病毒起着保护作用。然而，只有少量这种蛋白质已被确定。本文研究了烟粉虱亲环素B（CypB）在TYLCV蛋白质传播中的作用。研究结果表明，CypB在烟粉虱传播TYLCV中起着重要的作用。
外文摘要：Tomato yellow leaf curl virus (TYLCV) is a single-stranded (ssDNA) begomoviruses that causes severe damage to tomato and several other crops worldwide. TYLCV is exclusively transmitted by the sweetpotato whitefly, Bemisia tabaci in a persistent circulative and propagative manner. Previous studies have shown that the transmission, retention, and circulation of TYLCV in its vector involves interaction with insect and endosymbiont proteins, which aid in the transmission of the virus, or have a protective role in response to the presence of the virus in the insect body. However, only a low number of such proteins have been identified. Here, the role of B. tabaci Cyclophilin B (CypB) in the transmission of TYLCV protein was investigated. Cyclophilins are a large family of cellular prolyl isomerases that have many molecular roles including facilitating protein-protein interactions in the cell. One cyclophilin protein has been implicated in aphid-luteovirus interactions. We demonstrate that the expression of CypB from B. tabaci is altered upon TYLCV acquisition and retention. Further experiments used immunocapture-PCR and co-immunolocalization and demonstrated a specific interaction and colocalization between CypB and TYLCV in the the midgut, eggs, and salivary glands. Membrane feeding of anti-CypB antibodies and TYLCV-infected plants showed a decrease in TYLCV transmission, suggesting a critical role that CypB plays in TYLCV transmission. Further experiments, which used membrane feeding with the CypB inhibitor Cyclosporin A showed decrease in CypB-TYLCV colocalization in the midgut and virus transmission. Altogether, our results indicate that CypB plays an important role in TYLCV transmission by B. tabaci.
外文关键词：TYLCV；Bemisia tabaci；Cyclophilin B；immunostaining；virus transmission
作者：Kanakala, Surapathrudu；Ghanim, Murad
作者单位：Volcani Ctr, Dept Entomol, Rishon Leziyyon, Israel
期刊名称：FRONTIERS IN PLANT SCIENCE
期刊影响因子：4.495
出版年份：2016
出版刊次：11
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