中文摘要：节肢动物的唐氏综合征细胞粘附分子（Dscam）通过由选择性剪接产生的多种蛋白亚型来介导对病原菌的特异性识别。至今，大多数研究都集中在病原特异性免疫反应上，很少有人研究病毒或细菌细胞的进入。在本研究中，作者克隆和表征了灰飞虱的Dscam（LsDscam），并研究了LsDscam在水稻条纹病毒侵染中的作用及其对内共生菌沃尔巴克氏体的影响。研究结果表明：特定的Dscam异构体可能在增强病毒或内共生菌对介体的侵染中发挥了作用。
外文摘要：The arthropod Down syndrome cell adhesion molecule (Dscam) mediates pathogen-specific recognition via an extensive protein isoform repertoire produced by alternative splicing. To date, most studies have focused on the subsequent pathogen-specific immune response, and few have investigated the entry into cells of viruses or endosymbionts. In the present study, we cloned and characterized the cDNA of Laodelphax striatellus Dscam (LsDscam) and investigated the function of LsDscam in rice stripe virus (RSV) infection and the influence on the endosymbiont Wolbachia. LsDscam displayed a typical Dscam domain architecture, including 10 immunoglobulin (Ig) domains, six fibronectin type III domains, one transmembrane domain and a cytoplasmic tail. Alternative splicing occurred at the N-termini of the Ig2 and Ig3 domains, the complete Ig7 domain, the transmembrane domain and the C-terminus, comprising 10, 51, 35, two and two variable exons, respectively. Potentially LsDscam could encode at least 71 400 unique isoforms and 17 850 types of extracellular regions. LsDscam was expressed in various L. striatellus tissues. Knockdown of LsDscam mRNA via RNA interference decreased the titres of both RSV and Wolbachia, but did not change the numbers of the extracellular symbiotic bacterium Acinetobacter rhizosphaerae. Specific Dscam isoforms may play roles in enhancing the infection of vector-borne viruses or endosymbionts.
外文关键词：Down syndrome cell adhesion molecule；Laodelphax striatellus；rice stripe virus infection
作者：Zhang, F.；Li, Q.；Chen, X.；等
作者单位：中国科学院
期刊名称：INSECT MOLECULAR BIOLOGY
期刊影响因子：2.866
出版年份：2016
出版刊次：8
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