中文摘要：番茄黄化曲叶病是由不同的菜豆金色花叶病毒属病毒引起的，可导致全球番茄大量减产。在台湾，ToLCTWV和TYLCTHV是引起此病的主要菜豆金色花叶病毒属病毒种类。抗性基因可在野生番茄中获得，应对病毒菌株的快速进化需要持续寻找新的抗性基因和可替代控制的方法。RNA干扰是一种有效诱导抵抗病毒病原体抗性的技术。本研究结果表明，在番茄中构建的双生RNAi的表达可导致番茄对两种不同的菜豆金色花叶病毒属病毒的抗性。
外文摘要：Tomato yellow leaf curl disease caused by different begomovirus species leads to substantial tomato production losses worldwide. In Taiwan, the monopartite tomato leaf curl Taiwan virus (ToLCTWV) and bi-partite tomato yellow leaf curl Thailand virus (TYLCTHV) are the predominant begomovirus species causing this disease. Resistance genes are available in wild tomato species and a continuous search for new resistance genes and alternative control methods is required to respond to the rapid evolution of virus strains. RNA interference is an efficient technology to induce resistance against viral pathogens. Six different sections of the ToLCTWV genome were tested in transformed tomato for their capacity to reduce symptoms and inhibit viral DNA accumulation. The two most effective constructs for ToLCTWV infection carried regions of the C1 and C2 genes, and portions of either the C3 or C4 gene of ToLCTWV. A RNAi construct containing fusions of C1, C2 and C3 sections of ToLCTWV and the corresponding sections of the TYLCTHV DNA-A genome were introgressed into tomato line CLN1621L. R-1 and R-2 families were challenged using viruliferous whiteflies in separate screen houses for ToLCTWV and TYLCTHV. Sixteen and 12 R-2 plants derived from one primary transformant remained symptomless until at least 3 weeks after exposure to ToLCTWV and TYLCTHV, respectively, and accumulated only very low titres of viral DNA, as shown by real-time polymerase chain reaction analysis. Our results suggest that expression of bi-viral RNAi constructs in tomato can lead to resistance against two different tomato infecting begomovirus species.
外文关键词：Solanum lycopersicum; Transgenic resistance; Begomovirus; RNAi; Gene silencing
作者：Chen, Huei-mei; Lin, Chen-yu; Tsai, Wen-shi; 等
作者单位：台湾亚洲蔬菜研究发展中心
期刊名称： JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
期刊影响因子：1.352
出版年份：2016
出版刊次：4
点击下载：以两种菜豆金色花叶病毒属菌株为靶标的RNAi对番茄黄化曲叶病毒的抗性研究