中文摘要：水稻条纹病毒（RSV）是东亚地区最严重的水稻病毒之一。为了研究水稻如何响应RSV的侵染，本研究通过深度测序将miRNA表达与平行mRNA转录谱相结合。共鉴定了570个miRNA，其中有69个（5个上调，13个下调）明显被RSV侵染改变了。研究结果表明：宿主防御反应受RSV侵染选择性抑制；叶绿体功能也是RSV的目标，尤其是玉米黄质，这将影响类囊体膜的稳定性和ABA的生物合成。所有这些改变可能有助于病毒症状的发展，为RSV的致病机制提供了新的见解。
外文摘要：Rice stripe virus (RSV) is one of the most serious rice viruses in East Asia. To investigate how rice responds to RSV infection, we integrated miRNA expression with parallel mRNA transcription profiling by deep sequencing. A total of 570 miRNAs were identified of which 69 miRNAs (56 up-regulated and 13 down-regulated) were significantly modified by RSV infection. Digital gene expression (DGE) analysis showed that 1274 mRNAs (431 up-regulated and 843 down-regulated genes) were differentially expressed as a result of RSV infection. The differential expression of selected miRNAs and mRNAs was confirmed by qRT-PCR. Gene ontology (GO) and pathway enrichment analysis showed that a complex set of miRNA and mRNA networks were selectively regulated by RSV infection. In particular, 63 differentially expressed miRNAs were found to be significantly and negatively correlated with 160 target mRNAs. Interestingly, 22 up-regulated miRNAs were negatively correlated with 24 down-regulated mRNAs encoding disease resistance-related proteins, indicating that the host defense responses were selectively suppressed by RSV infection. The suppression of both osa-miR1423-5p- and osa-miR1870-5p-mediated resistance pathways was further confirmed by qRT-PCR. Chloroplast functions were also targeted by RSV, especially the zeaxanthin cycle, which would affect the stability of thylakoid membranes and the biosynthesis of ABA. All these modifications may contribute to viral symptom development and provide new insights into the pathogenicity mechanisms of RSV.
作者：Yang, Jian; Zhang, Fen; Li, Jing; 等
作者单位：浙江农业科学院
期刊名称：PLOS ONE
期刊影响因子：3.057
出版年份：2016
出版刊次：1
点击下载：通过对microRNAome和转录组的综合分析来阐释敏感水稻对水稻条纹病毒的反应